Abstract
We investigated the anti-inflammatory effects of minocycline in EAE, an animal model of MS. Minocycline, administered for two weeks after the clinical onset, significantly decreased the cumulative and mean clinical scores of EAE. This was associated with the reduction of both CD4(+) and CD8(+) T cell numbers in the spinal cord and the downregulation of LFA-1 on T cells without affecting the cytokine production profile. The predominant cytokine produced by T cells in the spleen was IFN-gamma whereas in the CNS it was IL-17. Our results indicate that minocycline regulates T cell infiltration into the CNS without modifying the dominant cytokine production.
Copyright 2009 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / metabolism
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Body Weight / drug effects
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Concanavalin A / pharmacology
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Cytokines / metabolism
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Disease Models, Animal
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Encephalomyelitis, Autoimmune, Experimental / drug therapy*
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Encephalomyelitis, Autoimmune, Experimental / immunology
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Encephalomyelitis, Autoimmune, Experimental / pathology*
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Female
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Flow Cytometry / methods
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Lymphocyte Function-Associated Antigen-1 / metabolism
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Minocycline / pharmacology
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Minocycline / therapeutic use*
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Rats
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Spinal Cord / metabolism
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Spinal Cord / pathology*
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Spinal Cord / physiopathology
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T-Lymphocytes / drug effects*
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T-Lymphocytes / metabolism
Substances
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Antigens, CD
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Cytokines
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Lymphocyte Function-Associated Antigen-1
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Concanavalin A
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Minocycline