In vivo brain imaging using a portable 2.9 g two-photon microscope based on a microelectromechanical systems scanning mirror

Opt Lett. 2009 Aug 1;34(15):2309-11. doi: 10.1364/ol.34.002309.

Abstract

We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Brain / blood supply*
  • Brain / cytology*
  • Capillaries / cytology*
  • Computer-Aided Design
  • Equipment Design
  • Equipment Failure Analysis
  • Lenses*
  • Mice
  • Micro-Electrical-Mechanical Systems / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Miniaturization
  • Reproducibility of Results
  • Sensitivity and Specificity