A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO)

C W Chang; T K Chou; R S Liu; S J Wang; W J Lin; C H Chen; H E Wang

doi:10.1016/j.apradiso.2007.01.005

A robotic synthesis of [18F]fluoromisonidazole ([18F]FMISO)

Appl Radiat Isot. 2007 Jun;65(6):682-6. doi: 10.1016/j.apradiso.2007.01.005. Epub 2007 Feb 6.

Authors

C W Chang¹, T K Chou, R S Liu, S J Wang, W J Lin, C H Chen, H E Wang

Affiliation

¹ Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, No. 155, Sec. 2, Li-Nong Street, Taipei 112, Taiwan.

PMID: 17379530
DOI: 10.1016/j.apradiso.2007.01.005

Abstract

This study aimed to develop an automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) using a Scanditronix Anatech RB III robotic system. [18F]HF was produced via the 18O(p,n)18F reaction using a Scanditronix MC17F cyclotron. On average, a typical run produced [18F]FMISO with an uncorrected radiochemical yield of 30+/-5% at end of synthesis (EOS) from the irradiation of 95% enriched [18O]water. The total synthesis time was 65 min. The retention time of [18F]FMISO (the radio-peak) was 4.9 min, which was consistent with the authentic FMISO (the ultraviolet peak). The radiochemical purity was greater than 97%. Preparation of [18F]FMISO using the automated robotic system is highly reliable and reproducible, and the radiation burden for the operator can be largely reduced. Sufficient radioactivities of [18F]FMISO could be obtained for non-invasive tumor hypoxia imaging in vivo with positron emission tomography (PET).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fluorine Radioisotopes / chemistry*
Hypoxia / diagnostic imaging
Misonidazole / analogs & derivatives*
Misonidazole / chemical synthesis
Neoplasms / diagnostic imaging
Neoplasms / metabolism
Positron-Emission Tomography
Radiopharmaceuticals / chemical synthesis*
Robotics

Substances

Fluorine Radioisotopes
Radiopharmaceuticals
fluoromisonidazole
Misonidazole