Abstract
We have evaluated the feasibility of human sodium iodide symporter (hNIS) as a reporter gene in vitro and in vivo. Recombinant adenovirus encoding hNIS (Rad-hNIS) was introduced to FRO cell for 48 hours. Western blotting and 99mTcO4 uptake study revealed functional hNIS expression in the cell. Rad-hNIS was injected to BALB/c mice via tail vein. 99mTcO4 gamma scintigraphy, biodistribution study, and RT-PCR analysis demonstrated a preferential hepatic uptake of 99mTcO4, which was observed for up to one week. Thus, hNIS can be utilized as an effective reporter gene for noninvasive/repeated imaging, in combination with 99mTcO4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics*
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Animals
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Gene Expression Regulation / genetics
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Genes, Reporter / genetics
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Humans
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Kidney / diagnostic imaging
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Kidney / embryology
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Kidney / metabolism*
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Male
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Metabolic Clearance Rate
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Mice
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Mice, Inbred BALB C
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Organ Specificity
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Radionuclide Imaging
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Radiopharmaceuticals / pharmacokinetics
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Recombinant Proteins / metabolism
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Sodium Pertechnetate Tc 99m / pharmacokinetics*
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Symporters / genetics
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Symporters / metabolism*
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Thyroid Gland / diagnostic imaging
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Thyroid Gland / metabolism*
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Tissue Distribution
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Transformation, Genetic / genetics
Substances
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Radiopharmaceuticals
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Recombinant Proteins
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Symporters
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sodium-iodide symporter
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Sodium Pertechnetate Tc 99m