Evaluation of desferal as a bifunctional chelating agent for labeling antibodies with Zr-89

W E Meijs; J D Herscheid; H J Haisma; H M Pinedo

doi:10.1016/0883-2889(92)90170-j

Evaluation of desferal as a bifunctional chelating agent for labeling antibodies with Zr-89

Int J Rad Appl Instrum A. 1992 Dec;43(12):1443-7. doi: 10.1016/0883-2889(92)90170-j.

Authors

W E Meijs¹, J D Herscheid, H J Haisma, H M Pinedo

Affiliation

¹ Radio-Nuclide-Centre (RNC), Free University, Amsterdam, The Netherlands.

PMID: 1334954
DOI: 10.1016/0883-2889(92)90170-j

Abstract

Zirconium-desferal was prepared and analysed by TLC, NMR and u.v.-spectroscopy. The stoichiometry of the complex was found to be 1:1. Chelation of desferal, coupled to resin, with 88Zr appeared to be fast and almost quantitative in various buffer systems in a broad pH-range (4-7). A high in vitro stability of the zirconium-desferal complex was observed; less than 0.2% zirconium was lost within 24 h in plasma-solutions.

MeSH terms

Antibodies*
Chelating Agents*
Deferoxamine*
Evaluation Studies as Topic
Isotope Labeling / methods*
Radioisotopes*
Zirconium*

Substances

Antibodies
Chelating Agents
Radioisotopes
Zirconium
Deferoxamine