Abstract
In rats, the relationship between [(18)F]FHPG accumulation and HSVtk expression was studied with PET and autoradiography. [(18)F]FHPG distribution closely corresponded with HSVtk immunohistochemical staining. ROI analysis of tracer uptake 2 hours p.i. and Patlak graphical analysis were applied to quantify the PET data. For both analysis methods, the [(18)F]FHPG PET signal correlated well with the fraction of HSVtk expressing cells implanted, but showed a plateau when plotted against HSVtk protein levels. This might be due to rate limiting [(18)F]FHPG membrane transport.
Publication types
-
Comparative Study
-
Evaluation Study
-
Research Support, Non-U.S. Gov't
-
Validation Study
MeSH terms
-
Animals
-
Ganciclovir / analogs & derivatives*
-
Ganciclovir / pharmacokinetics*
-
Gene Expression Profiling / methods
-
Gene Expression Regulation, Neoplastic*
-
Glioma / diagnostic imaging*
-
Glioma / genetics
-
Glioma / metabolism*
-
Metabolic Clearance Rate
-
Radiopharmaceuticals / pharmacokinetics
-
Rats
-
Rats, Nude
-
Reproducibility of Results
-
Sensitivity and Specificity
-
Statistics as Topic
-
Thymidine Kinase / genetics
-
Thymidine Kinase / metabolism*
-
Tomography, Emission-Computed / methods*
-
Whole-Body Counting
Substances
-
9-((3-fluoro-1-hydroxy-2-propoxy)methyl)guanine
-
Radiopharmaceuticals
-
Thymidine Kinase
-
Ganciclovir