MR microscopy of magnetically labeled neurospheres transplanted into the Lewis EAE rat brain

Magn Reson Med. 2003 Jul;50(1):201-5. doi: 10.1002/mrm.10511.

Abstract

Stem cell transplantation is being explored as a new paradigm for the treatment of demyelinating diseases. Magnetically labeled multipotential neural precursor cells were transplanted into the ventricles of rats with acute experimental allergic encephalomyelitis (EAE) and high-resolution (microscopic) MR images were obtained ex vivo. Migration patterns of live cells into periventricular white matter structures could be easily visualized, with a good correlation of the corresponding histopathology. The present results confirm that MR cell tracking can be used to guide the development of successful transplantation protocols.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Validation Study

MeSH terms

  • Animals
  • Brain / pathology*
  • Brain / surgery
  • Cell Movement
  • Contrast Media
  • Encephalomyelitis, Autoimmune, Experimental / pathology*
  • Encephalomyelitis, Autoimmune, Experimental / physiopathology
  • Encephalomyelitis, Autoimmune, Experimental / surgery*
  • Female
  • Ferrosoferric Oxide
  • Iron
  • Magnetic Resonance Spectroscopy / instrumentation*
  • Magnetic Resonance Spectroscopy / methods*
  • Microscopy / instrumentation
  • Microscopy / methods
  • Microspheres
  • Neurons / pathology
  • Oxides
  • Rats
  • Rats, Inbred Lew
  • Staining and Labeling / instrumentation
  • Staining and Labeling / methods
  • Stem Cell Transplantation / instrumentation
  • Stem Cell Transplantation / methods*
  • Stem Cells / pathology*

Substances

  • Contrast Media
  • Oxides
  • Iron
  • Ferrosoferric Oxide