Cytokine expression in three mouse models of experimental hepatitis

Cytokine. 2002 Aug 7;19(3):115-20. doi: 10.1006/cyto.2002.1948.

Abstract

The activation of T-cells and macrophages and subsequent induction of cytokines are critical factors in the development of hepatitis. Up-regulation of pro-inflammatory cytokines, e.g. TNF has been shown to induce liver injury while counter regulation by anti-inflammatory cytokines, e.g. IL-10 is protective. We compared the induction of liver injury and the expression pattern of a variety of cytokines in T-cell- versus non-T-cell-dependent mouse models of liver injury. TNF, IFNgamma, IL-2, IL-4, IL-6, IL-10 and IL-12 were measured in plasma and liver tissue after either Concanavalin A (Con A), D-galactosamine/lipopolysaccharide (GalN/LPS) or high dose LPS induced liver injury. Additionally, the intra-hepatic expression of the putative pathogenicity factor high mobility group 1 protein (HMG-1) was compared in all three models.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Concanavalin A / pharmacology
  • Cytokines / biosynthesis*
  • Cytokines / blood*
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Galactosamine / pharmacology
  • Hepatitis / blood*
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / biosynthesis
  • Interleukin-12 / biosynthesis
  • Interleukin-2 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Interleukin-6 / biosynthesis
  • Kinetics
  • Lipopolysaccharides / pharmacology
  • Liver / injuries
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Up-Regulation

Substances

  • Cytokines
  • Interleukin-2
  • Interleukin-6
  • Lipopolysaccharides
  • Tumor Necrosis Factor-alpha
  • Concanavalin A
  • Interleukin-10
  • Interleukin-12
  • Interleukin-4
  • Galactosamine
  • Interferon-gamma

Associated data

  • GENBANK/J03783
  • GENBANK/M28621
  • GENBANK/M37897
  • GENBANK/M86672
  • GENBANK/X02611
  • GENBANK/X03765