Abstract
The activation of T-cells and macrophages and subsequent induction of cytokines are critical factors in the development of hepatitis. Up-regulation of pro-inflammatory cytokines, e.g. TNF has been shown to induce liver injury while counter regulation by anti-inflammatory cytokines, e.g. IL-10 is protective. We compared the induction of liver injury and the expression pattern of a variety of cytokines in T-cell- versus non-T-cell-dependent mouse models of liver injury. TNF, IFNgamma, IL-2, IL-4, IL-6, IL-10 and IL-12 were measured in plasma and liver tissue after either Concanavalin A (Con A), D-galactosamine/lipopolysaccharide (GalN/LPS) or high dose LPS induced liver injury. Additionally, the intra-hepatic expression of the putative pathogenicity factor high mobility group 1 protein (HMG-1) was compared in all three models.
Copyright 2002 Elsevier Science Ltd.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Concanavalin A / pharmacology
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Cytokines / biosynthesis*
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Cytokines / blood*
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Disease Models, Animal
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Enzyme-Linked Immunosorbent Assay
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Galactosamine / pharmacology
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Hepatitis / blood*
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Interferon-gamma / biosynthesis
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Interleukin-10 / biosynthesis
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Interleukin-12 / biosynthesis
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Interleukin-2 / biosynthesis
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Interleukin-4 / biosynthesis
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Interleukin-6 / biosynthesis
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Kinetics
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Lipopolysaccharides / pharmacology
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Liver / injuries
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Reverse Transcriptase Polymerase Chain Reaction
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Time Factors
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Tumor Necrosis Factor-alpha / biosynthesis
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Up-Regulation
Substances
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Cytokines
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Interleukin-2
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Interleukin-6
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Lipopolysaccharides
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Tumor Necrosis Factor-alpha
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Concanavalin A
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Interleukin-10
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Interleukin-12
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Interleukin-4
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Galactosamine
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Interferon-gamma
Associated data
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GENBANK/J03783
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GENBANK/M28621
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GENBANK/M37897
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GENBANK/M86672
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GENBANK/X02611
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GENBANK/X03765