Expression of oncogene products HER2/Neu and Ras and fibrosis-related growth factors bFGF, TGF-beta, and PDGF in bile from biliary malignancies and inflammatory disorders

W C Su; S C Shiesh; H S Liu; C Y Chen; N H Chow; X Z Lin

doi:10.1023/a:1010619316436

Expression of oncogene products HER2/Neu and Ras and fibrosis-related growth factors bFGF, TGF-beta, and PDGF in bile from biliary malignancies and inflammatory disorders

Dig Dis Sci. 2001 Jul;46(7):1387-92. doi: 10.1023/a:1010619316436.

Authors

W C Su¹, S C Shiesh, H S Liu, C Y Chen, N H Chow, X Z Lin

Affiliation

¹ Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

PMID: 11478488
DOI: 10.1023/a:1010619316436

Abstract

The expression of several growth factors and K-ras gene mutation in bile were studied to better understand the pathogenesis and improve early diagnosis of bile duct cancers. Bile samples were collected from 12 cholangiocarcinomas (CLC), 10 ampullary cancers (APC), 3 gallbladder cancers (GBC), 7 pancreatic cancers (PNC), 9 biliary tract infection (BTI), 8 biliary stone disease (ST), and 5 normal controls (NC). The highest mean value of TGF-beta in bile was in patients with BTI; the mean levels of bFGF and PDGF were highest in CLC, and patients with APC and CLC had higher expression of HER2/Neu than other groups. In bile, a K-ras gene codon 12 mutation was found in 5 of 6 (83%) cases of CLC by the PCR-RFLP method. The results suggest overexpression of bFGF, PDGF, and HER2/Neu and the presence of K-ras mutation are important for carcinogenesis of bile duct cancers, and detection of the above abnormalities in bile is helpful for early diagnosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ampulla of Vater
Bile / chemistry*
Bile Duct Neoplasms / diagnosis
Bile Duct Neoplasms / metabolism
Bile Ducts, Intrahepatic
Biliary Tract Diseases / diagnosis
Biliary Tract Diseases / metabolism*
Biliary Tract Neoplasms / diagnosis
Biliary Tract Neoplasms / metabolism*
Biomarkers / analysis
Biomarkers, Tumor / analysis
Cholangiocarcinoma / diagnosis
Cholangiocarcinoma / metabolism
Cholelithiasis / diagnosis
Cholelithiasis / metabolism*
Common Bile Duct Neoplasms / diagnosis
Common Bile Duct Neoplasms / metabolism
Enzyme-Linked Immunosorbent Assay
Gallbladder Neoplasms / diagnosis
Gallbladder Neoplasms / metabolism
Humans
Infections / metabolism
Pancreatic Neoplasms / diagnosis
Pancreatic Neoplasms / metabolism
Platelet-Derived Growth Factor / analysis*
Polymerase Chain Reaction
Receptor, ErbB-2 / analysis*
Transforming Growth Factor beta / analysis*
ras Proteins / analysis*

Substances

Biomarkers
Biomarkers, Tumor
Platelet-Derived Growth Factor
Transforming Growth Factor beta
Receptor, ErbB-2
ras Proteins