Up-regulation of the peripheral-type benzodiazepine receptor expression and [(3)H]PK11195 binding in gerbil hippocampus after transient forebrain ischemia

J Neurosci Res. 2001 Jun 1;64(5):493-500. doi: 10.1002/jnr.1101.

Abstract

In mammalian CNS, the peripheral-type benzodiazepine receptor (PTBR) is localized on the outer mitochondrial membrane within the astrocytes and microglia. The main function of PTBR is to transport cholesterol across the mitochondrial membrane to the site of neurosteroid biosynthesis. The present study evaluated the changes in the PTBR density, gene expression and immunoreactivity in gerbil hippocampus as a function of reperfusion time after transient forebrain ischemia. Between 3 to 7 days of reperfusion, there was a significant increase in the maximal binding site density (B(max)) of the PTBR antagonist [(3)H]PK11195 (by 94-156%; P < 0.01) and PTBR mRNA levels (by 1.8- to 2.9-fold; P < 0.01). At 7 days of reperfusion, in the hippocampal CA1 (the brain region manifesting selective neuronal death), PTBR immunoreactivity increased significantly. Increased PTBR expression after transient forebrain ischemia may lead to increased neurosteroid biosynthesis, and thus may play a role in the ischemic pathophysiology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology*
  • Binding Sites / drug effects
  • Binding Sites / physiology
  • Brain Ischemia / metabolism*
  • Brain Ischemia / pathology
  • Brain Ischemia / physiopathology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology
  • Gerbillinae
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • Hippocampus / physiopathology
  • Immunohistochemistry
  • Isoquinolines / pharmacokinetics*
  • Male
  • Mitochondria / metabolism
  • Mitochondria / ultrastructure
  • Nerve Degeneration / metabolism
  • Nerve Degeneration / physiopathology
  • Neuroglia / metabolism
  • Neuroglia / ultrastructure
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, GABA-A / genetics
  • Receptors, GABA-A / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Reperfusion Injury / metabolism*
  • Reperfusion Injury / pathology
  • Reperfusion Injury / physiopathology
  • Steroids / biosynthesis
  • Tritium / pharmacokinetics
  • Up-Regulation / drug effects*
  • Up-Regulation / physiology

Substances

  • Antineoplastic Agents
  • Isoquinolines
  • RNA, Messenger
  • Receptors, GABA-A
  • Recombinant Fusion Proteins
  • Steroids
  • Tritium
  • PK 11195