Abstract
Annexin V was radiolabelled with iodine-123 in order to develop a SPECT-ligand for imaging atherosclerosis and apoptosis. Iodination by means of electrophylic substitution resulted in radiochemical yields up to 70% and specific activities of 7.4-92.5 MBq/microg protein. Binding experiments with blood platelets indicated that 123I-labelled annexin V remained its biological activity.
MeSH terms
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Annexin A5 / chemical synthesis*
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Blood Platelets / metabolism
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Chromatography, High Pressure Liquid
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Dose-Response Relationship, Drug
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Humans
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Iodine Radioisotopes / chemistry
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Ligands
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Protein Binding
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Recombinant Proteins / chemical synthesis*
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Time Factors
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Tomography, Emission-Computed, Single-Photon / methods
Substances
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Annexin A5
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Iodine Radioisotopes
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Ligands
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Recombinant Proteins