Synthesis and in vitro evaluation of 123I-labelled human recombinant annexin V

C Lahorte; G Slegers; J Philippé; C Van de Wiele; R A Dierckx

doi:10.1016/s1389-0344(00)00062-9

Synthesis and in vitro evaluation of 123I-labelled human recombinant annexin V

Biomol Eng. 2001 Jan;17(2):51-3. doi: 10.1016/s1389-0344(00)00062-9.

Authors

C Lahorte¹, G Slegers, J Philippé, C Van de Wiele, R A Dierckx

Affiliation

¹ Department of Radiopharmacy, Faculty of Pharmaceutical Sciences, Harelbekestraat 72, B-9000, Ghent, Belgium. christophe.lahorte@rug.ac.be

PMID: 11163751
DOI: 10.1016/s1389-0344(00)00062-9

Abstract

Annexin V was radiolabelled with iodine-123 in order to develop a SPECT-ligand for imaging atherosclerosis and apoptosis. Iodination by means of electrophylic substitution resulted in radiochemical yields up to 70% and specific activities of 7.4-92.5 MBq/microg protein. Binding experiments with blood platelets indicated that 123I-labelled annexin V remained its biological activity.

MeSH terms

Annexin A5 / chemical synthesis*
Blood Platelets / metabolism
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Humans
Iodine Radioisotopes / chemistry
Ligands
Protein Binding
Recombinant Proteins / chemical synthesis*
Time Factors
Tomography, Emission-Computed, Single-Photon / methods

Substances

Annexin A5
Iodine Radioisotopes
Ligands
Recombinant Proteins