Abstract
A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [(11)C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Chromatography, High Pressure Liquid / instrumentation
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Chromatography, High Pressure Liquid / methods*
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Cocaine / analogs & derivatives*
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Cocaine / blood
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Dopamine Agents / blood
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Imidazoles / blood
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Isoquinolines / blood
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Ligands
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Pyridines / blood
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Raclopride / blood
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Sensitivity and Specificity
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Solvents / chemistry
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Tetrabenazine / analogs & derivatives
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Tetrabenazine / blood
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Time Factors
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Tomography, Emission-Computed*
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Urea / chemistry
Substances
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Dopamine Agents
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Imidazoles
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Isoquinolines
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Ligands
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Pyridines
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Solvents
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L 159884
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dihydrotetrabenazine
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Raclopride
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(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester
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Urea
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McN 5652
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Cocaine
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Tetrabenazine