Abstract
To provide long-term therapy in patients with severe toxin-induced hepatic parenchymal damage, donor hepatocytes would need to replicate and replace a large portion of the damaged parenchyma. Using a mouse model developed to reproduce this type of hepatic injury, we found that hepatocyte transplantation only slightly improved survival after transplantation despite the fact that many non-survivors showed moderate liver repopulation by donor cells. Perhaps accounting for this outcome, donor parenchyma in non-survivors did not have typical lobular organization. These results indicate that the re-creation of functional parenchyma by transplanted hepatocytes requires time, during which donor cells proliferate and then establish normal parenchymal architecture.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkaline Phosphatase / genetics
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Animals
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Cell Transplantation*
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Disease-Free Survival
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Enhancer Elements, Genetic
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Ganciclovir / toxicity*
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Genetic Therapy
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Herpesvirus 1, Human / enzymology
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Herpesvirus 1, Human / genetics
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Humans
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Liver / cytology*
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Liver / drug effects
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Liver / pathology
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Liver Neoplasms, Experimental / chemically induced
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Liver Neoplasms, Experimental / pathology*
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Liver Neoplasms, Experimental / therapy*
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Metallothionein / genetics
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Mice
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Mice, Transgenic
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / biosynthesis
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Serum Albumin / genetics
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Thymidine Kinase / genetics
Substances
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Recombinant Fusion Proteins
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Serum Albumin
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Metallothionein
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Thymidine Kinase
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Alkaline Phosphatase
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Ganciclovir