Radiolabelled pyrimidine nucleosides to monitor the expression of HSV-1 thymidine kinase in gene therapy

Nucleosides Nucleotides. 1999 Apr-May;18(4-5):1065-6. doi: 10.1080/15257779908041646.

Abstract

Selective radiolabelling and imaging of transduced HSV tk expressing cells was studied using [123I]IVFRU, [125I]FIRU and [125I]FIAU. Although all three radionucleosides accumulated in the KBALB-STK transduced murine tumour line in vitro and in vivo, [125I]FIRU provided optimal performance in terms of selectivity for HSV tk expressing cells and % of injected dose accumulating in the tumor.

MeSH terms

  • Animals
  • Genetic Therapy*
  • Herpesvirus 1, Human / enzymology*
  • Iodine Radioisotopes
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Neoplasms, Experimental / metabolism
  • Neoplasms, Experimental / therapy*
  • Pyrimidine Nucleosides / metabolism*
  • Thymidine Kinase / genetics*
  • Tissue Distribution
  • Tumor Cells, Cultured

Substances

  • Iodine Radioisotopes
  • Pyrimidine Nucleosides
  • Thymidine Kinase