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Photoacoustic and Fluorescence Image-Guided Surgery Using a Multifunctional Targeted Nanoprobe

  • Breast Oncology
  • Published:
Annals of Surgical Oncology Aims and scope Submit manuscript

Abstract

Purpose

A complete surgical excision with negative tumor margins is the single most important factor in the prediction of long-term survival for most cancer patients with solid tumors. We hypothesized that image-guided surgery using nanoparticle-enhanced photoacoustic and fluorescence imaging could significantly reduce the rate of local recurrence.

Methods

A murine model of invasive mammary carcinoma was utilized. Three experimental groups were included: (1) control; (2) tumor-bearing mice injected with non-targeted nanoprobe; and (3) tumor-bearing mice injected with targeted nanoprobe. The surgeon removed the primary tumor following the guidance of photoacoustic imaging (PAI), then inspected the surgical wound and removed the suspicious tissue using intraoperative near-infrared (NIR) fluorescence imaging. The mice were followed with bioluminescence imaging weekly to quantify local recurrence.

Results

Nanoprobe-enhanced photoacoustic contrast enabled PAI to map the volumetric tumor margins up to a depth of 31 mm. The targeted nanoparticles provided significantly greater enhancement than non-targeted nanoparticles. Seven mice in the group injected with the targeted nanoprobes underwent additional resections based upon NIR fluorescence imaging. Pathological analysis confirmed residual cancer cells in the re-resected specimens in 5/7 mice. Image-guided resection resulted in a significant reduction in local recurrence; 8.7 and 33.3 % of the mice in the targeted and control groups suffered recurrence, respectively.

Conclusions

These results suggest that photoacoustic and NIR intraoperative imaging can effectively assist a surgeon to locate primary tumors and to identify residual disease in real-time. This technology has promise to overcome current clinical challenges that result in the need for second surgical procedures.

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Acknowledgment

We thank Dr. Andrew Y. Wang at Ocean Nanotech, LLC, for providing magnetic IONPs, and Dr. Malgorzata Lipowska at Emory University for synthesis of NIR 830 dye. This research project was supported by the following National Institutes of Health (NIH) Grants: NIH R21CA 161384 (Huabei Jiang) and R01CA133722 (Lily Yang).

Disclosures

No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA

    Lei Xi PhD & Huabei Jiang PhD

  2. Department of Surgery, University of Florida, Gainesville, FL, USA

    Guangyin Zhou PhD & Steven J. Hughes MD

  3. Department of Surgery, Emory University School of Medicine, Atlanta, GA, USA

    Ning Gao PhD & Lily Yang MD, PhD

  4. Department of Pathology, University of Florida, Gainesville, FL, USA

    David A. Gonzalo MD

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  1. Lei Xi PhD
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  2. Guangyin Zhou PhD
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Correspondence to Huabei Jiang PhD.

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Xi, L., Zhou, G., Gao, N. et al. Photoacoustic and Fluorescence Image-Guided Surgery Using a Multifunctional Targeted Nanoprobe. Ann Surg Oncol 21, 1602–1609 (2014). https://doi.org/10.1245/s10434-014-3541-9

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  • DOI: https://doi.org/10.1245/s10434-014-3541-9

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