Issue 7, 2005
From the journal:

Lab on a Chip

Imaging of peptideadsorption to microfluidic channels in a plastic compact disc using a positron emitting radionuclide

Martin Lavén,a   Irina Velikyan,b   Majda Djodjic,a   Jenny Ljung,a   Oskar Berglund,a   Karin Markides,*a   Bengt Långströmbc  and  Susanne Wallenborgd  

* Corresponding authors

a Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, Box 599, 751 24 Uppsala, Sweden
E-mail: karin.markides@kemi.uu.se

b Department of Organic Chemistry, Institute of Chemistry, Uppsala University, Box 599, 751 24 Uppsala, Sweden

c Uppsala Imanet AB, Box 967, 751 09 Uppsala, Sweden

d Gyros AB, Uppsala Science Park, 751 83 Uppsala, Sweden

Abstract

A method for studying peptide–surface interactions within microfluidic channels by radionuclide imaging is described. With the high surface area-to-volume ratio of channels in miniaturised devices, combined with low amounts of analyte, non-specific peptide adsorption is a critical issue. The objective of the study was therefore to develop a method capable of direct detection of adsorbed peptide within microfluidic channels. A micro-device consisting of channels moulded in a plastic compact disc was chosen for the study, together with two selected peptides of different lengths and isoelectric point (pI) values. A bifunctional chelator, DOTA, was attached to the peptide by conjugation and labelled with the short-lived positron emitting radionuclide 68Ga. Quantitative images of radiotracer distribution within the microfluidic channels were obtained using a PhosphorImager system. The power of the method was demonstrated by the ability to clearly measure changes in adsorption when varying a number of parameters that typically affect peptide adsorption. These included surface modifications, analyte concentration, pH, and ionic strength. Additionally, two quantification methods were developed and compared. Radionuclide imaging also permitted visualisation of adsorption and release processes in microchannel chromatographic columns. The results suggest that radionuclide imaging is a suitable tool not only for the study of peptide adsorption to the microchannels presented in this study but also as a versatile tool to measure peptide–surface interactions in a wide variety of miniaturised structures and devices.

Graphical abstract: Imaging of peptide adsorption to microfluidic channels in a plastic compact disc using a positron emitting radionuclide

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Article information

DOI
https://doi.org/10.1039/B418715F
Article type
Paper
Submitted
13 Dec 2004
Accepted
10 May 2005
First published
31 May 2005

Lab Chip, 2005,5, 756-763

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Imaging of peptide adsorption to microfluidic channels in a plastic compact disc using a positron emitting radionuclide

M. Lavén, I. Velikyan, M. Djodjic, J. Ljung, O. Berglund, K. Markides, B. Långström and S. Wallenborg, Lab Chip, 2005, 5, 756 DOI: 10.1039/B418715F

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