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Complete reversal of multidrug resistance by stable expression of small interfering RNAs targeting MDR1

Gene Therapy volume 11, pages 1170–1174 (2004)Cite this article

Abstract

Overexpression of P-glycoprotein, encoded by the MDR1 gene, confers multidrug resistance (MDR) on cancer cells and is a frequent impediment to successful chemotherapy. Recent developments in the use of small interfering RNAs to inhibit specific protein expression have highlighted their potential use as therapeutic agents. We have expressed two different short hairpin RNAs from stably integrated plasmids in doxorubicin-resistant K562 leukaemic cells. The MDR1-targeted RNA interference (RNAi) resulted in decreased MDR1 mRNA, abolished P-glycoprotein expression, and completely reversed the MDR phenotype to that of the drug-sensitive K562 parental line. This study demonstrates that MDR, which is solely due to overexpression of P-glycoprotein, can be reversed by RNAi. These target sequences can in the future be integrated into gene therapy vectors with potential clinical application.

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Acknowledgements

This work was supported by the Medical Research Council UK. We thank Ms Katie Smith for assistance with flow cytometry.

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  1. Medical Research Council Clinical Sciences Centre, Imperial College Faculty of Medicine, Hammersmith Hospital Campus, Du Cane Road, London, UK

    E Yagüe, C F Higgins & S Raguz

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  1. E Yagüe
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  3. S Raguz
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Yagüe, E., Higgins, C. & Raguz, S. Complete reversal of multidrug resistance by stable expression of small interfering RNAs targeting MDR1. Gene Ther 11, 1170–1174 (2004). https://doi.org/10.1038/sj.gt.3302269

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