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Fusion protein from RGD peptide and Fc fragment of mouse immunoglobulin G inhibits angiogenesis in tumor

Cancer Gene Therapy volume 11, pages 363–370 (2004)Cite this article

Abstract

Targeting tumor vasculature represents an interesting approach for the treatment of solid tumors. The αvβ3 integrins have been found to be specifically associated with angiogenesis in tumors. By using bacteriophage display technology, Ruoslahti et al found that a group of peptides containing the RGD (Arg-Gly-Asp) motif have high-binding affinity to the αvβ3 integrins in tumors. In this study, we designed a fusion protein containing the RGD sequence and the Fc fragment of mouse IgG in order to target the Fc portion of IgG to the tumor vasculature to elicit an antiangiogenesis immune response. In vivo angiogenesis and tumor studies demonstrated that the fusion protein (RGD/mFc) inhibited tumor angiogenesis and tumor growth and improved overall survival. This approach may generate new therapeutic agents for solid tumor treatment.

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Acknowledgements

We thank Eric Holle for his work in animal care and maintenance, and Lakendra Workman for her expert secretarial assistance. This work was supported in part by the Oncology Research Foundation of the Greenville Hospital System, Greenville, SC, USA

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Authors and Affiliations

  1. Oncology Research Institute, Greenville Hospital System, Greenville, 29605, SC, USA

    Jinhua Li, Lillia M Holmes, Kelly E Burgin, Lori B Barton, Xianzhong Yu, Thomas E Wagner & Yanzhang Wei

  2. Department of Biological Sciences, Clemson University, Clemson, 29634, SC, USA

    Jianfei Ji, Xianzhong Yu, Thomas E Wagner & Yanzhang Wei

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  1. Jinhua Li
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  2. Jianfei Ji
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  3. Lillia M Holmes
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Correspondence to Yanzhang Wei.

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Li, J., Ji, J., Holmes, L. et al. Fusion protein from RGD peptide and Fc fragment of mouse immunoglobulin G inhibits angiogenesis in tumor. Cancer Gene Ther 11, 363–370 (2004). https://doi.org/10.1038/sj.cgt.7700707

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