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A discrete sequence in a platelet integrin is involved in ligand recognition

Nature volume 350, pages 66–68 (1991)Cite this article

Abstract

PLATELET membrane glycoprotein Ilb-IIIa (gpIIb-IIIa; αllb-β3), the most prominent member of the integrin family of adhesion receptors on these cells, mediates platelet aggregation by binding fibrinogen and is critical in thrombosis and haemostasis1-5. A short amino-acid sequence at the carboxy terminus of the γ chain of fibrinogen is recognized by gpIIb-IIIa (ref. 6) and peptides containing this sequence are selectively crosslinked to residues 294-314 of gpllb (ref. 7). Here we show that an 11-residue peptide from this region of gpllb inhibits platelet aggregation and binding of fibrinogen to platelets and to purified gpIIb-IIIa, and that it interacts directly with fibrinogen. These results implicate this segment of gpIIb-IIIa in the ligand-binding function of the receptor. Moreover, as this region is highly conserved among integrins, it may have a general function in ligand recognition by this broadly distributed family of adhesion receptors.

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References

  1. Hynes, R. O. Cell 48, 549–554 (1987).

    Article  CAS  PubMed  Google Scholar 

  2. Phillips, D. R., Charo, I. F., Parise, L. V. & Fitzgerald, L. A. Blood 71, 831–843 (1988).

    CAS  PubMed  Google Scholar 

  3. Plow, E. F. & Ginsberg, M. H. in Progress in Hemostasis and Thrombosis Vol. 9 (ed. Coller, B.S.) 117–156 (Saunders, Philadelphia, 1989).

    Google Scholar 

  4. Yasuda, T. et al. J. clin. Invest. 81, 1284–1291 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Akiyama, S. K., Nagata, K. & Yamada, K. M. Biochim. biophys. Acta 1031, 91–110 (1990).

    Article  CAS  PubMed  Google Scholar 

  6. Kloczewiak, M., Timmons, S., Lukas, T. J. & Hawiger, J. Biochemistry 23, 1767–1774 (1984).

    Article  CAS  PubMed  Google Scholar 

  7. D'Souza, S. E., Ginsberg, M. H., Burke, T. A. & Plow, E. F. J. biol. Chem. 265, 3440–3446 (1990).

    CAS  PubMed  Google Scholar 

  8. Fitzgerald, L. A., Steiner, B., Rall, S. C. Jr, Lo, S.-S. & Phillips, D. R. J. biol. Chem. 262, 3936–3939 (1987).

    CAS  PubMed  Google Scholar 

  9. Poncz, M. et al. J. biol. Chem. 262, 8476–8482 (1987).

    CAS  PubMed  Google Scholar 

  10. Kretsinger, R. H. & Nockolds, C. E. J. biol. Chem. 248, 3313–3326 (1973).

    CAS  PubMed  Google Scholar 

  11. Matsueda, G. R., Ball, E. L. & Bernatowicz, M. S. FASEB J. 2, 6480 (1988).

    Google Scholar 

  12. Plow, E. F., Pierschbacher, M. D., Ruoslahti, E., Marguerie, G. A. & Ginsberg, M. H. Proc. natn. Acad. Sci. U.S.A. 82, 8057–8061 (1985).

    Article  ADS  CAS  Google Scholar 

  13. Ginsberg, M. H., Pierschbacher, M. D., Ruoslahti, E., Marguerie, G. & Plow, E. J. biol. Chem. 260, 3931–3936 (1985).

    CAS  PubMed  Google Scholar 

  14. Haverstick, D. M., Cowan, J. F., Yamada, K. M. & Santoro, S. A. Blood 66, 946–952 (1985).

    CAS  PubMed  Google Scholar 

  15. Gartner, T. K. & Bennett, J. S. J. biol. Chem. 260, 11891–11894 (1985).

    CAS  PubMed  Google Scholar 

  16. Parise, L. V. & Phillips, D. R. J. biol. Chem. 260, 10698–10707 (1985).

    CAS  PubMed  Google Scholar 

  17. Lam, C-T. et al. J. biol. Chem. 262, 947–950 (1987).

    CAS  PubMed  Google Scholar 

  18. Bennett, J. S., Shattil, S. J., Power, J. W. & Gartner, T. K. J. biol. Chem. 263, 12948–12953 (1988).

    CAS  PubMed  Google Scholar 

  19. D'Souza, S. E., Ginsberg, M. H., Burke, T. A., Lam, S. C-T. & Plow, E. F. Science 242, 91–93 (1988).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Loftus, J. C. et al. Science 249, 915–918 (1990).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Charo, I. F., Nannizzi, L., Phillips, D. R., Hsu, M. A. & Scarborough, R. M. Blood 74, 497a (1989).

    Google Scholar 

  22. Kobayashi, T., Takagi, T., Konishi, K. & Wnuk, W. J. biol. Chem. 264, 18247–18259 (1989).

    CAS  PubMed  Google Scholar 

  23. Hemler, M. E. A. Rev. Immun. 8, 365–400 (1990).

    Article  CAS  Google Scholar 

  24. Ginsberg, M. H. et al. J. biol. Chem. 262, 5437–5440 (1987).

    CAS  PubMed  Google Scholar 

  25. Yamada, K. M. & Kennedy, D. W. J. cell. Physiol. 130, 21–28 (1987).

    Article  CAS  PubMed  Google Scholar 

  26. Akiyama, S. K. & Yamada, K. M. J. biol. Chem. 260, 10402–10405 (1985).

    CAS  PubMed  Google Scholar 

  27. Marguerie, G. A., Edgington, T. S. & Plow, E. F. J. biol. Chem. 255, 154 (1980).

    CAS  PubMed  Google Scholar 

  28. Plow, E. F., Pierschbacher, M. D., Ruoslahti, E., Marguerie, G. & Ginsberg, M. H. Blood 70, 110–115 (1987).

    CAS  PubMed  Google Scholar 

  29. Pytela, R., Pierschbacher, M. D., Ginsberg, M. H., Plow, E. F. & Ruoslahti, E. Science 231, 1559–1562 (1986).

    Article  ADS  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Committee on Vascular Biology, Research Institute of Scripps Clinic, 10666 North Torrey Pines Road, La Jolla, California, 92037, USA

    Stanley E. D'Souza, Mark H. Ginsberg & Edward F. Plow

  2. Squibb Laboratories/Biology, Princeton University, Princeton, New Jersey, 08544, USA

    Gary R. Matsueda

Authors
  1. Stanley E. D'Souza
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  2. Mark H. Ginsberg
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  3. Gary R. Matsueda
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  4. Edward F. Plow
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D'Souza, S., Ginsberg, M., Matsueda, G. et al. A discrete sequence in a platelet integrin is involved in ligand recognition. Nature 350, 66–68 (1991). https://doi.org/10.1038/350066a0

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