Abstract
[18F]FLT (3′-deoxy-3′-[18F]fluorothymidine) turned out to be a tracer particularly suitable for PET imaging of tumor proliferation because of lacking degradation in vivo. To facilitate clinical studies with [18F]FLT, we investigated two new easily accessible precursors, 2,3′-anhydrothymidine (AThy) and 5′-O-(4,4′-dimethoxytriphenylmethyl)-2,3′-anhydrothymidine (DMTThy), using a common approach for introducing the label with nucleophilic [18F]fluoride. Radiochemical yields were determined in dependence on substrate concentration, reaction time and temperature. In the case of AThy (10 mg), best FLT yields were 5.3%±1.2 (130 °C, 30 min). Labeling of DMTThy (10 mg) gave 14.3%±3.3 at 160 °C within 10 minutes. Starting with an aqueous solution of 20 GBq [18F]fluoride the new method allows to produce 1.3 GBq [18F]FLT within 90 minutes ready for intravenous injection. The new labeling procedures allow [18F]FLT synthesis without lengthy preparation of the precursor and with high reproducibility mandatory for clinical application.
Similar content being viewed by others
References
J. R. Grierson, A. F. Shields, J. F. Eary, J. Labeled Comp. Radiopharm., 40 (1997) 60.
J. R. Grierson, A. F. Shields, J. Nucl. Med., 40 (1999) 83.
J. R. Grierson, A. F. Shields, J. Labeled Comp. Radiopharm., 42(Suppl. 1) (1999) 525.
J. R. Grierson, A. F. Shields, B. M. Dohmen et al., Nature Medicine, 4 (1998) 1334.
H. Siegmund, W. Pfleiderer, Helv. Chim. Acta, 79 (1996) 426.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Machulla, HJ., Blocher, A., Kuntzsch, M. et al. Simplified Labeling Approach for Synthesizing 3′-Deoxy-3′-[18F]fluorothymidine ([18F]FLT). Journal of Radioanalytical and Nuclear Chemistry 243, 843–846 (2000). https://doi.org/10.1023/A:1010684101509
Issue Date:
DOI: https://doi.org/10.1023/A:1010684101509