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A History of the First Uncoupling Protein, UCP1

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Abstract

The lack of energy conservation in brown adipose tissue mitochondria when prepared byconventional methods was established in the 1960s and was correlated with the thermogenicfunction of the tissue. In order to observe energy conservation, two requirements had to bemet: the removal of the endogenous fatty acids and the addition of a purine nucleotide. Thesetwo factors have been the essential tools that led to the discovery of the energy dissipationpathway, the uncoupling protein UCP1. The activity is regulated by these two ligands. Purinenucleotides bind from the cytosolic side of the protein and inhibit transport. Fatty acids actas seconds messengers of noradrenaline and increase the proton conductance. This reviewpresents a historical perspective of the steps that led to the discovery of UCP1, its regulation,and our current view on its mechanism of transport.

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REFERENCES

  • Bouillaud, F., Casteilla, L., and Ricquier, D. (1992). Mol. Biol. Evol. 9, 970-975.

    Google Scholar 

  • Bouillaud, F., Arechaga, I., Petit, P. X., Raimbault, S., Levi-Meyrueis, C., Casteilla, L., Laurent, M., Rial, E., and Ricquier, D. (1994). EMBO J. 13, 1990-1997.

    Google Scholar 

  • Cunningham, S. A., Wiesinger, H., and Nicholls, D. G. (1986). Eur. J. Biochem. 157, 415-420.

    Google Scholar 

  • Dierks, T., Stappen, R., and Krámer, R. (1994). In Molecular Biology of Mitochondrial Transport Systems(Forte, M., and Colombini, M., eds.), Springer-Verlag, Berlin, pp. 117-129.

    Google Scholar 

  • Flatmark, T., and Pedersen, J. I. (1975). Biochim. Biophys. Acta 416, 53-103.

    Google Scholar 

  • Foster, D. O., and Frydman, L. (1978). Can. J. Physiol. Pharmacol. 56, 110-122.

    Google Scholar 

  • Garlid, K. D., Orosz, D. E., Modriansky, M., Vassanelli, M., and Jezek, P. (1996) J. Biol. Chem. 271, 2615-2620.

    Google Scholar 

  • Garlid, K. D., Jaburek, M., and Jezek, P. (1998). FEBS Lett. 438, 10-14.

    Google Scholar 

  • González-Barroso, M. M., Fleury, C., Arechaga, I., Zaragoza, P., Levi-Meyrueis, C., Raimbault, S., Bouillaud, F., and Rial, E. (1996). Eur. J. Biochem. 239, 445-450.

    Google Scholar 

  • González-Barroso, M. M., Fleury, C., Levi-Meyrueis, C., Zaragoza, P., Bouillaud, F., and Rial, E. (1997). Biochemistry 36, 10930-10935.

    Google Scholar 

  • González-Barroso, M. M., Fleury, C., Bouillaud, F., Nicholls, D. G., and Rial, E. (1998). J. Biol. Chem. 273, 15528-15532.

    Google Scholar 

  • Hamilton, J. A., and Cistola, D. P. (1986). Proc. Natl. Acad. Sci. USA 83, 82-86.

    Google Scholar 

  • Hittelman, K. J., Lindberg, O., and Cannon, B. (1969). Eur. J. Biochem. 11, 183-192.

    Google Scholar 

  • Heaton, G. M., and Nicholls, D. G. (1976). Eur. J. Biochem. 67, 511-517.

    Google Scholar 

  • Heaton, G. M., and Nicholls, D. G. (1977). Biochem. Soc. Trans. 5, 210-212.

    Google Scholar 

  • Heaton, G. M., Wagenvoord, R. J., Kemp, A., and Nicholls, D. G. (1978). Eur. J. Biochem. 82, 515-521.

    Google Scholar 

  • Krämer, R. (1998). Exp. Physiol. 83, 259-265.

    Google Scholar 

  • Klingenberg, M., and Winkler, E. (1985). EMBO J. 4, 3087-3092.

    Google Scholar 

  • Lin, C. S., and Klingenberg, M. (1982). Biochemistry 21, 2950-2956.

    Google Scholar 

  • Locke, R. M., and Nicholls, D. G. (1981). FEBS Lett. 135, 249-252.

    Google Scholar 

  • Locke, R. M., Rial, E., Scott, I. D., and Nicholls, D. G. (1982a). Eur. J. Biochem. 129, 373-380.

    Google Scholar 

  • Locke, R. M., Rial, E., and Nicholls, D. G. (1982b). Eur. J. Biochem. 129, 381-387.

    Google Scholar 

  • Nicholls, D. G. (1974a). Eur. J. Biochem. 49, 573-583.

    Google Scholar 

  • Nicholls, D. G. (1974b). Eur. J. Biochem. 49, 585-593.

    Google Scholar 

  • Nicholls, D. G. (1974c). Eur. J. Biochem. 50, 305-315.

    Google Scholar 

  • Nicholls, D. G. (1976). Eur. J. Biochem. 62, 223-228.

    Google Scholar 

  • Nicholls, D. G. (1977). Eur. J. Biochem. 77, 349-356.

    Google Scholar 

  • Nicholls, D. G. (1979). Biochim. Biophys. Acta 549, 1-29.

    Google Scholar 

  • Nicholls, D. G., and Heaton, G. M. (1978). In The Proton and Calcium Pumps(Azzone, A., Awron, M., Metcalfe, J. C., Quagliariello, E., and Siliprandi, N, eds.), Elsevier, Amsterdam, pp.309-318.

    Google Scholar 

  • Nicholls, D. G., and Lindberg, O. (1972). FEBS Lett. 25, 61-64.

    Google Scholar 

  • Nicholls, D. G., and Lindberg, O. (1973). Eur. J. Biochem. 37, 523-530.

    Google Scholar 

  • Nicholls, D. G., and Locke, R. M. (1984). Physiol. Rev. 64, 1-64.

    Google Scholar 

  • Nicholls, D. G., Grav, H. J., and Lindberg, O. (1972). Eur. J. Biochem. 31, 526-533.

    Google Scholar 

  • Nicholls, D. G., Snelling, R., and Rial, E. (1984). Biochem. Soc. Trans. 12, 388-390.

    Google Scholar 

  • Prusiner, S. B., Cannon, B., Ching, T. M., and Lindberg, O. (1968). Eur. J. Biochem. 7, 51-57.

    Google Scholar 

  • Rafael, J., and Heldt, H. W. (1976). FEBS Lett. 63, 304-308.

    Google Scholar 

  • Rafael, J., Ludolph, H.-J., and Hohorst, H.-J. (1969). Hoppe-Seyler's Z. Physiol. Chem. 350, 1121-1131.

    Google Scholar 

  • Rafael, J., Fesser, W., and Nicholls, D. G. (1986). Amer. J. Physiol. 250, C228-C235.

    Google Scholar 

  • Rial, E., and Nicholls, D. G. (1986). Eur. J. Biochem. 161, 689-694.

    Google Scholar 

  • Rial, E., Poustie, A., and Nicholls, D. G. (1983). Eur. J. Biochem. 137, 197-203.

    Google Scholar 

  • Rial, E., Arechaga, I., Sainz-de-la-Maza, E., and Nicholls, D. G. (1989). Eur. J. Biochem. 182, 187-193.

    Google Scholar 

  • Rial, E., González-Barroso, M. M., Fleury, C., and Bouillaud, F. (1998). Biofactors 8, 209-219.

    Google Scholar 

  • Ricquier, D., and Kader, J. C. (1976). Biochem. Biophys. Res. Commun. 73, 577-583.

    Google Scholar 

  • Skulachev, V. P. (1991). FEBS Lett. 294, 158-162.

    Google Scholar 

  • Smith, R. E., and Horwitz, B. A. (1969). Physiol. Rev. 49, 330-425.

    Google Scholar 

  • Trayhurn, P., and Nicholls, D. G., eds. (1986). In Brown Adipose Tissue, Edward Arnold, London.

    Google Scholar 

  • Viguera, A. R., Goñi, F. M., and Rial, E. (1992). Eur. J. Biochem. 210, 893-899.

    Google Scholar 

  • Walker, J. E., and Runswick, M. J. (1993). J. Bioenerg. Biomembr. 25, 435-446.

    Google Scholar 

  • Winkler, E., and Klingenberg, M. (1994). J. Biol. Chem. 269, 2508-2515.

    Google Scholar 

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Correspondence to Eduardo Rial.

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Nicholls, D.G., Rial, E. A History of the First Uncoupling Protein, UCP1. J Bioenerg Biomembr 31, 399–406 (1999). https://doi.org/10.1023/A:1005436121005

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