PET imaging of hypoxiaHypoxia imaging with the nitroimidazole 18F-FAZA PET tracer: A comparison with OxyLite, EPR oximetry and 19F-MRI relaxometry
Section snippets
Animal and tumor model
Male adult WAG/Rij rats (Harlan Nederland, Horst, The Netherlands) weighting between 200 and 250 g were used for this study. Fragments of about 1 mm3 from a syngenic rhabdomyosarcoma were grafted subcutaneously in both thighs of each rat under general anesthesia by using intraperitoneal injection of a mixed solution of ketamine and xylazine (80 and 10 mg/kg, respectively). The experiments were performed when tumors grew to be 20–25 mm in diameter (at this size of tumor, the proportion of necrotic
Comparison between 18F-FAZA PET imaging and OxyLite oximetry
In this comparison (Fig. 2), animals were transferred to OxyLite system immediately after the PET scans. The mean ± SEM pO2 measured by OxyLite for the tumors used in normoxic condition was 1.89 ± 0.60 mm Hg, n = 11. A significant increase of this value was observed in the tumors of carbogen group (pO2 = 7.12 ± 1.74 mm Hg, n = 13). Similarly for PET imaging, 18F-FAZA exhibited a significant lower uptake in the case of carbogen (T/B ratio = 2.18 ± 0.71) than that of room air (T/B ratio = 2.90 ± 0.75). The best trend
Discussion
Considering the important role of tumor oxygenation, many hypoxia-targeting therapies have emerged and are being developed [35]. These therapeutic strategies, however, cannot be fully utilized unless we have a suitable method to assess hypoxia in routine clinical procedure. The current standard method [36] (Eppendorf measurements) is invasive and cannot provide three-dimensional heterogeneity of oxygenation. Moreover, these systems are not produced anymore by Eppendorf, and, consequently this
Acknowledgements
This work is supported by grants from Belgian National Fund for Scientific Research (FNRS), Televie, Fonds Joseph Maisin, Saint-Luc Foundation, Fondation contre le Cancer, “Actions de Recherches Concertées-Communauté Française de Belgique-ARC 09/14-020”.
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