Micro-PET in rodents
Immobilization device for in vivo and in vitro multimodality image registration of rodent tumors

https://doi.org/10.1016/j.radonc.2008.02.016Get rights and content

Abstract

Biological image-guided radiotherapy requires that PET accurately identifies biologically relevant sub-volumes within a tumor. In this framework, an immobilization device was developed to study multi-imaging (CT, micro-MRI, micro-PET, and autoradiography) registration of mouse tumors. The registration accuracy assessed by calculating the average minimal distance between two skew lines was in the order of 0.2–0.3 mm.

Section snippets

Animal and tumor model

Eight to 10-week-old male C3H/HeOuJIco (IFFA Credo Belgium) mice were used in this study. Animals were maintained in a facility approved by the Belgian Ministry of Agriculture in accordance with current regulations and standards. Animals were housed 4–5 per cage and fed ad libitum. FSA II fibrosarcoma syngeneic to C3Hf/Kam mice were generated in the right thigh. These tumors were kindly provided by Dr. L. Milas from the University of Texas, M.D. Anderson Cancer Center, Houston, USA. Maintenance

Results and discussion

The alignment of the various autoradiography slices was quite accurate with an average root mean square error of 0.2 mm. The registration accuracy between the various imaging modalities including the autoradiography was also very good. On average, translation misalignment reached 0.2 mm for all four pairs of comparisons (Table 1). The rotation error was also very low, reaching a maximum of 3.5° for PET–autoradiography registration.

Our data compare well with other repositioning devices for small

Acknowledgements

Nicolas Christian is a research fellow of the “Fonds pour la formation à la Recherche dans l’Industrie et l’Agriculture (F.R.I.A.)” of Belgium. The project was supported by a grant from the “Fonds Joseph Maisin”, Brussels, Belgium, by the European Commission’s Sixth Framework Programme funding (Contract No. LSHC-CT-2004-505785), and by a program project from the “Institut National du Cancer” of France (Project INCa N° RS 020).

References (11)

There are more references available in the full text version of this article.

Cited by (0)

View full text