Micro-PET in rodentsImmobilization device for in vivo and in vitro multimodality image registration of rodent tumors
Section snippets
Animal and tumor model
Eight to 10-week-old male C3H/HeOuJIco (IFFA Credo Belgium) mice were used in this study. Animals were maintained in a facility approved by the Belgian Ministry of Agriculture in accordance with current regulations and standards. Animals were housed 4–5 per cage and fed ad libitum. FSA II fibrosarcoma syngeneic to C3Hf/Kam mice were generated in the right thigh. These tumors were kindly provided by Dr. L. Milas from the University of Texas, M.D. Anderson Cancer Center, Houston, USA. Maintenance
Results and discussion
The alignment of the various autoradiography slices was quite accurate with an average root mean square error of 0.2 mm. The registration accuracy between the various imaging modalities including the autoradiography was also very good. On average, translation misalignment reached 0.2 mm for all four pairs of comparisons (Table 1). The rotation error was also very low, reaching a maximum of 3.5° for PET–autoradiography registration.
Our data compare well with other repositioning devices for small
Acknowledgements
Nicolas Christian is a research fellow of the “Fonds pour la formation à la Recherche dans l’Industrie et l’Agriculture (F.R.I.A.)” of Belgium. The project was supported by a grant from the “Fonds Joseph Maisin”, Brussels, Belgium, by the European Commission’s Sixth Framework Programme funding (Contract No. LSHC-CT-2004-505785), and by a program project from the “Institut National du Cancer” of France (Project INCa N° RS 020).
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