Elsevier

Neuroscience Letters

Volume 458, Issue 1, 10 July 2009, Pages 28-31
Neuroscience Letters

In vivo micro-CT imaging of rat brain glioma: A comparison with 3 T MRI and histology

https://doi.org/10.1016/j.neulet.2009.04.033Get rights and content

Abstract

The aim of this study was to evaluate the potential of a novel micro-CT system to image in vivo the extent of tumor in a rat model of malignant glioma compared to 3 T magnetic resonance imaging (MRI) and histology. Fourteen animals underwent double dose contrast-enhanced imaging with micro-CT and 3 T MRI using a clinical machine at day 10 after stereotactic F98 glioma cell implantation. Calculation of the volume of the contrast-uptaking part of the tumor was done by manually outlining the tumor contours by two experienced neuroradiologists. The micro-CT- and MRI-derived tumor volumes were compared to histology as gold standard (hematoxylin and eosin staining and fluorescence staining). There was high interobserver reability regarding the tumor volumes (Crombach's α > 0.81). Also, there was good correlation of micro-CT- and high-field MRI-derived tumor volumes compared to histology: 72 ± 21 mm3 and 69 ± 23 mm3 compared to 81 ± 14 mm3, respectively (r > 0.76). Both the micro-CT- and MRI-derived tumor volumes were not significantly smaller compared to histology (P > 0.14). In conclusion, micro-CT allows in vivo imaging of the contrast-enhancing part of experimental gliomas with an accuracy comparable to high-field MRI.

References (21)

There are more references available in the full text version of this article.

Cited by (31)

  • Micro-imaging of Brain Cancer Radiation Therapy Using Phase-contrast Computed Tomography

    2018, International Journal of Radiation Oncology Biology Physics
    Citation Excerpt :

    Brain tumor angiogenesis can also be studied using micro-MR angiography, at spatial resolutions of 60 μm and after contrast agent administration (8). Conventional x-ray micro-CT is able to assess the presence of tumors in small animals in vivo (9) after contrast media injection but, otherwise, affords soft tissue sensitivity that is, in absence of contrast agents, rather low. Ex vivo high-resolution MRI after active staining (10) allows for the study of full-organ brain tissue microstructure down to 10- to 25-μm isotropic resolution, quantitative regional volumetric measurements, and the elaboration of several distinct image contrasts, so-called proton stains, to highlight specific anatomic features.

  • Comparison between perfusion computed tomography and dynamic contrast-enhanced magnetic resonance imaging in assessing glioblastoma microvasculature

    2017, European Journal of Radiology
    Citation Excerpt :

    Therefore, the two contrast agents may have different diffusion behaviors in the examined tissue [18]. Some reports have compared CT and MRI in assessing glioma microvasculature [19–21]. For instance, De Simone M et al. compared cerebral blood volume (CBV) obtained by PCT and first-pass dynamic susceptibility-weighted contrast-enhanced T2-weighted magnetic resonance imaging (DSC-MRI) data, and found that both modalities showed proportional results in CBV assessment of the patients with high grade glioma [19].

  • Micro-computed tomography in murine models of cerebral cavernous malformations as a paradigm for brain disease

    2016, Journal of Neuroscience Methods
    Citation Excerpt :

    Unlike osmium tetroxide and other contrast agents (Metscher, 2009a; White et al., 1976), the iodine technique (Metscher, 2009b; Wong et al., 2013) does not render the tissue unusable after the staining thus allowing continuation of investigation with histology on the same tissue. The micro-CT technique has previously been utilized to image brain diseases (Dobrivojevic et al., 2013; Engelhorn et al., 2009; Park et al., 2014) including recent report with our group showing feasibility in imaging CCM in murine models (Zhou et al., 2016). However, those studies did not include direct validation in relation to histologic morphometry, in part because the osmium staining techniques applied previously do not preserve the histologic structure for direct correlations.

  • Usefulness of clinical magnetic resonance scanners for imaging experimental changes in laboratory rodents' central nervous system

    2012, Polish Annals of Medicine
    Citation Excerpt :

    Imaging parameters are presented in Table 1. A clinical Tim Trio scanner (Siemens, Erlangen, Germany) and orbital surface coil with a diameter of 40 mm were used to visualize glioma in rats.8 In this study, 14 female Fisher rats, weighing 150–200 g, were injected with the F98 tumor cell line.

View all citing articles on Scopus
View full text