Original articleMultispectral Imaging Autofluorescence Microscopy in Colonic and Gastric Cancer Metastatic Lymph Nodes
Section snippets
Lymph Node Sampling: Recruitment and Treatment of Lymph Nodes
For this study, we enrolled a total of 30 patients: 15 affected by colorectal cancer and 15 affected by gastric cancer. Two control lymph nodes were obtained from informed nonneoplastic patients.
We expressly chose neoplastic patients who were in the advanced stages of the disease and who were candidates for adjuvant therapy. In these patients, the removal of suspected metastatic lymph nodes, independent of location, did not affect the individual patient’s scheduled procedure. None of the
Results
Both multicolored imaging and spectroscopic techniques were used to characterize the AF properties of metastatic lymph nodes and to distinguish them from normal lymph nodes.
Biopsy sections of the lymph nodes, prepared as described in the Materials and Methods section, were processed by AMS and MIAM, sequentially.
Comparison between the AF spectra of metastatic lymph nodes and the control lymph nodes (Figure 1) revealed meaningful differences: the maximum had shifted toward red by more than 20
Discussion
In the past years, the technologic improvements in the fields of excitation sources, light delivery systems, and, in particular, the increase of device sensitivity for the detection and analysis of fluorescence signals, together with a better knowledge of the photo physical characteristics of the endogenous fluorophores, have opened interesting perspectives for applying AF in the characterization of animal tissue features and consequently in diagnosis.
The present study shows that AF analysis
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Supported in part by the Ministero dell’Istruzione della Università e della Ricerca Scientifica grant (2002064155).