Basic Science and Experimental StudiesHypercholesterolemia Exacerbates Ventricular Remodeling in the Rat Model of Myocardial Infarction
Section snippets
Study Design and Induction of MI
The study was conducted in accordance with the Guide for the Care and Use of Laboratory Animals (US National Institutes of Health Publication No. 85-23, revised 1985) and was approved by the Ethics Committee of our institution.
Male 6-week-old Sprague-Dawley rats were randomly assigned to normal chow (normocholesterolemic) or high-cholesterol chow (hypercholesterolemic) (n = 31 per group) and were maintained on such diets for 8 weeks before induction of MI and until the end of the experiment.
Results
Table 1 shows baseline data for both hypercholesterolemic and normocholesterolemic rats. Baseline echocardiography (Fig. 2) revealed no differences between these 2 groups.
Of 31 normocholesterolemic rats subjected to left coronary ligation, 2 animals died within first 3 hours and additional 4 between 3 hours and 24 hours after the procedure. In high-cholesterol group, these figures were 3 and 5, respectively. All 14 animals that died within first 24 hours after the procedure had WMI ≤15
Discussion
In the present study, we show that diet-induced hypercholesterolemia exacerbates LV remodeling and heart failure in the rat model of nonreperfused myocardial infarction and that mechanism of this phenomenon is unrelated to effect of hypercholesterolemia on MI size. This observation adds new dimension to the recognized risk factor of coronary artery disease—hypercholesterolemia. Our results suggest that apart from its well known effects on coronary plaque progression and stability it can worsen
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2021, Biomedicine and PharmacotherapyCitation Excerpt :We used echocardiography to estimate ischemic area and infarct area. Although we have previously shown that results of such estimation closely match results of histological analysis [33], this is a limitation of the study. We did not analyze iron status in this study, although in a previous study in a similar post-MI rat model we demonstrated normal systemic and cardiac iron status of the rat [3].
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2018, Canadian Journal of CardiologyCitation Excerpt :Under light anesthesia (ketamine HCl and xylazine, 75 and 3.5 mg/kg body weight, i.p.), contractility of 12 wall segments visualized in the midpapillary short-axis view and 11 segments visualized in the long-axis view was graded as 1 (normal) or 0 (abnormal), and the total wall motion index (WMI) was calculated. As we have previously shown, WMI closely correlated with infarct size and that WMI = 15 corresponded to infarct size ∼ 40%.8 LV end-diastolic and end-systolic areas were planimetered from the parasternal long-axis view.
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2010, Journal of Molecular and Cellular CardiologyCitation Excerpt :Normal hearts had WMI = 23. Our previous results revealed that WMI closely correlated with infarct size and that WMI = 15 corresponded to infarct size ∼40% [15]. LV ejection fraction (LVEF) was calculated as (LV diastolic area − LV systolic area)/LV diastolic area, both planimetered from the parasternal long-axis view.
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2010, American Journal of CardiologyLate ventricular remodeling in non-reperfused acute myocardial infarction in humans is predicted by angiotensin II type 1 receptor density on blood platelets
2008, International Journal of CardiologyCitation Excerpt :The only significant differences were that LDL cholesterol was higher and diabetes was more prevalent in the high AT1R group. Since in our population [7] and in previous studies [8] AT1R were shown to correlate with LDL cholesterol and LDL cholesterol has been suggested to deteriorate post-MI LV remodeling [21,22], we correlated it with indices of LV remodeling. We found no correlation between LDL cholesterol and indices of LV dilation in either univariate or multivariate analysis.
Supported by a grant of Medical Center of Postgraduate Education 501-2-1-27-97/03 and a grant of Polish Ministry of Science 2 P05A02827.