Elsevier

Brain Research

Volume 1039, Issues 1–2, 28 March 2005, Pages 199-202
Brain Research

Short communication
MicroPET detection of enhanced 18F-FDG utilization by PKA inhibitor in awake rat brain

https://doi.org/10.1016/j.brainres.2005.01.064Get rights and content

Abstract

To obtain PET imaging of glucose metabolism in the brains of conscious rats, a method of rat head fixation was developed. PET measurement with microPET was performed for 60 min after 18F-FDG injection. Significant enhancement of glucose utilization in the right striatum was observed with infusion of Rp-adenosine-3,5-cyclic phosphorothioate triethylamine (Rp-cAMPS). FDG uptake increments were also seen in the ipsilateral frontal cortex and thalamus. As initial FDG uptake in the brain was not significantly altered by Rp-cAMPS, increased glucose metabolism might be due to an increase in the phosphorylation rate by hexokinase rather than the delivery process from plasma to the brain. In contrast to awake rats, the effect of Rp-cAMPS was abolished by anesthesia using chloral hydrate, indicating that neuronal activity has an important role in short term regulation of hexokinase activity through the cAMP/PKA system in the brain. These results strongly demonstrated the value of measuring glucose utilization in the brains of conscious rats.

References (10)

There are more references available in the full text version of this article.

Cited by (42)

  • Molecular Imaging: A New Frontier in Neurotoxicology

    2017, Comprehensive Toxicology: Third Edition
  • A voxel-based analysis of brain activity in high-order trigeminal pathway in the rat induced by cortical spreading depression

    2015, NeuroImage
    Citation Excerpt :

    Because FDG is taken up and remains within activated regions during conscious conditions, it can be imaged at the end of the uptake period for a short time while the animal is under anesthesia (Jang et al., 2009; Schiffer et al., 2007; Sung et al., 2009; Thompson and Bushnell, 2012). It is well known that anesthesia affects various neural responses, including pain transmission and perception (Hosoi et al., 2005; Onoe et al., 1994). In the present study, we successfully revealed brain activity in the conscious rat and identified activated brain regions in response to cortical SD, which may display migraine pain transmission and perception.

  • Towards a reproducible protocol for repetitive and semi-quantitative rat brain imaging with <sup>18</sup> F-FDG: Exemplified in a memantine pharmacological challenge

    2014, NeuroImage
    Citation Excerpt :

    Small animal 18 F-FDG PET allows us to study glucose metabolism longitudinally in vivo in laboratory animals (Backes et al., 2011; Dedeurwaerdere et al., 2005; Fueger et al., 2006a; Hosoi et al., 2005; Jang et al., 2009; Lee et al., 2005; Nguyen et al., 2012; Sung et al., 2009; Wong et al., 2011).

  • Molecular imaging in neuroscience research with small-animal PET in rodents

    2011, Neuroscience Research
    Citation Excerpt :

    To overcome the shortcomings of anesthesia, a few studies have been carried out to image conscious rodents by using a head-fixation device. Hosoi et al. (2005) developed a head-fixation system that has proved valuable for measuring glucose metabolism in the conscious rat brain. Recently, Mizuma et al. (2010) developed an in vivo imaging system in conscious mice, using a head-fixation device, to perform quantitative kinetic analysis of regional cerebral glucose metabolic rate.

View all citing articles on Scopus
View full text