Imidazol-1-ylalkanoic acids as extrinsic 1H NMR probes for the determination of intracellular pH, extracellular pH and cell volume
Imidazol-1-ylalkanoate esters I have been prepared by reaction of imidazole with α-bromo esters or with α,β-unsaturated esters. The corresponding acids have been obtained by hydrolysis. Esters and acids have been evaluated as extrinsic 1H NMR probes for the determination of intracellular pH, extracellular pH and cell volume in rat erythrocytes.
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