A detailed mapping of the histamine H3 receptor and its gene transcripts in rat brain
Section snippets
Tissue preparation
All experiments performed in the present study conformed to the National Institutes of Health guidelines (décret no. 2001-464, May 29, 2001, from the French Ministry of Agriculture). Male Wistar rats (Iffa-Credo, L’Arbresle, France) were killed by decapitation, their brain was removed rapidly, immediately frozen (−40°C) by immersion in monochlorodifluoromethane and stored at −70°C. Brain sections (10 μm) were prepared on a cryostat and thaw-mounted onto Superfrost slides. For in situ
Results
The densities of [125I]iodoproxyfan binding sites and H3R mRNAs in the various structures were quantified and/or arbitrarily rated and the results are given in Fig. 1 and Table 1, Table 2, Table 3, Table 4, Table 5.
Autoradiograms from sagittal or frontal sections generated with [125I]iodoproxyfan or with a selective antisense riboprobe are shown in Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6. Fig. 7, Fig. 8, Fig. 9 display the cellular localization of H3R mRNAs in sections from various brain areas.
Discussion
The aim of the present work was to rate and compare the detailed autoradiographic distributions of the H3R and its gene transcripts on adjacent brain sections from the same rats, using two selective probes, i.e. the radioligand [125I]iodoproxyfan (Ligneau et al., 1994) and a 33P-labelled riboprobe corresponding to a partial coding sequence (∼600 bp) of the rat H3R and detecting all the previously cloned functional isoforms of the receptor (Morisset et al., 2001, Héron et al., 2001). The
Conclusion
The present study provides evidence for the expression of H3Rs upon many neuronal cell types. If some localizations are consistent with previous functional studies, the physiological role of many of the receptors present on neuronal perikarya, dendrites and/or projections remains to be understood. Moreover, many of these localizations do not parallel the histaminergic innervation, and the question then arises as to how these receptors are activated. We showed recently that native H3Rs in rodent
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The histamine H3 receptor modulates dopamine D2 receptor–dependent signaling pathways and mouse behaviors
2023, Journal of Biological ChemistryThe prefrontal cortex shows widespread decrease in H3 histamine receptor binding densities in rats with genetic generalized epilepsies
2022, Epilepsy ResearchCitation Excerpt :The minimal level of significance was set at p = 0.05. In general, the distribution of the H3 histamine receptor densities observed in the present study (Figs. 1 and 2) was similar to the earlier reported studies (Pollard et al., 1993; Pillot et al., 2002). The prefrontal cortex demonstrated dense binding both in its medial and lateral subdivisions (Figs. 1,2).
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These authors contributed equally to this study.