Neutrophil labeling: Problems and pitfalls

doi:10.1016/S0001-2998(84)80024-0

Seminars in Nuclear Medicine

Volume 14, Issue 2, April 1984, Pages 107-117

https://doi.org/10.1016/S0001-2998(84)80024-0 Get rights and content

The use of neutrophils labeled with gamma-emitting radionuclides has been shown to be acceptable for in vivo kinetic studies as well as for imaging inflammatory foci. Among the gamma-emitting radionuclides, indium-111 appears to be the agent of choice. Labeling neutrophils with ¹¹¹In, however, is a relatively new technique. Although simple to perform, it involves several stages, none of which could be carried out without problems. These are discussed and the current research aimed at eliminating the problems is outlined. The knowledge of specific chemotactic receptors and surface antigens has stimulated investigations into selective neutrophil labeling that will continue to be challenging and exciting.

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Neutrophil labeling: Problems and pitfalls

J Immunol Methods

Blood

Int J Appl Radiat Isot

J Surg Res

Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents

J Nucl Med

Indium-111-labeled leukocytes for the localization of abscesses: Preparation, analysis, tissue distribution, and comparison with gallium-67 citrate in dogs

J Lab Clin Med

The Neutrophil

Leukokinetic studies: The total blood circulation and marginal granulocyte pools and the granulocyte turnover rate in normal subjects

J Clin Invest

Separation of lymphocytes, polymorphonuclear leukocytes on glass columns including tissue culture observations

Blood

Glass wool separation of rat polymorphonuclear leukocytes according to adherence and maturity

j Reticuloendothel Sov

Isolation of mononuclear cells and granulocytes from human blood

Scand J Clin Lab Invest

Comparison of H-3-di-isopropyl fluorophosphate and P-32-Di-isopropyl Fluorosphosphate for neutrophil kinetic studies

Acta Haematol

Indium-111-labeled autologous leukocoytes in man

J Nucl Med

Cr-51 and DFP-32 labeling of human blood cells in leukocyte-rich plasma

Exp Hematol

A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using the modification of the Hypaque/Ficoll technique

J Immunol Methods

Glass wool separation of rat polymorphonuclear leukocytes according to adherence and maturity

J Reticuloendothel Soc

Isolation of large numbers of fully viable human neutrophils: a preparative technique using Percoll density gradient centrifugation

Exp Hematol

Technique for increased granulocyte recovery from human whole blood by counterflow centrifugation-elutriation. I. In vitro analysis

Transfusion

The role of radiopharmaceuticals in nuclear hematology

Radiopharmaceuticals II: Proceedings of the Second International Symposium on Radiopharmaceuticals

Cell Labeling: Achievements, challenges, and prospects

J Nucl Med

In vitro labeling of bone marrow and leukemic blood leukocytes with tritiated thymidine

J Lab Clin Med

Selenium-75 seleno-methionine as a label for erythrocytes, leukocytes, and platelets in man

J Lab Clin Med

Scintigraphic demonstration of experimental abscesses with intravenous Ga-67-citrate and Ga-67 labeled blood leukocytes

Ann Surg

Ga-67 or In-111 white blood cell scan for abcess detection: A case for In-111

Clin Nucl Med

Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I.

Particles J Nucl Med

Five leukocyte labeling techniques: A comparative in vitro study

Br J Haematol

Cell labeling with colloidal substances in whole blood

Eur J Nucl Med

Inflammatory lesions localized with technetium Tc 99m-labeled leukocytes

Arch Intern Med

Indium-111 labeled leukocytes for the localization of abscess, preparation, analysis, tissue distribution and comparison with gallium-67 citrate in dogs

J Lab Clin Med

Indium-111 labeled cellular blood components: Mechanism of labeling and intracellular location in human neutrophils

J Nucl Med

Indium-111-labeled human polymorphonuclear leukocytes: Viability, random migration, chemotaxis, bactericidal capacity, and ultrastructure

J Nucl Med