The use of 2-[18F]fluoro-2-deoxy-d-glucose as a potential in vitro agent for labelling human granulocytes for clinical studies by positron emission tomography

doi:10.1016/0883-2897(92)90006-K

International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology

Volume 19, Issue 2, February 1992, Pages 183-190

International Journa...

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Abstract

In this study, 2-[¹⁸F]fluoro-2-deoxy-d-glucose, ([¹⁸F]FDG) was used to radiolabel human granulocytes in vitro for possible clinical use by positron emission tomography (PET). Uptake of [¹⁸F]FDG was dependent on the amount of glucose in the labelling medium, e.g. when 1 × 10⁷ granulocytes were incubated with [¹⁸F]FDG containing 15μg/mL glucose 80% of [¹⁸F]FDG was incorporated within 30 min, but in the presence of 1 mg/mL of glucose it was reduced to 2%. Increasing the cell concentration and activating the granulocytes with Streptococcus pneumoniae, opsonized zymosan or phorbol myristate acetate all increased the uptake of [¹⁸F]FDG. Retention of the [¹⁸F]FDG by the cells as [¹⁸F]FDG-6-phosphate was also dependent on the extracellular glucose concentration, 9% was released within 60 min in the absence of glucose, but 27% in the presence of 1 mg/mL glucose.

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International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology

Abstract

References (18)

Basic methods for the study of phagocytosis

Methods Enzymol.

Measurement of neutrophil function: phagocytosis, degranulation, the respiratory burst and bacterial killing

In vivo measurement of the local metabolic response to lung inflammation in rabbits by positron emission tomography (PET)

Clin. Sci.

Optimum conditions for radiolabelling human granulocytes and mixed leucocytes with ¹¹¹In-tropolonate

Eur. J. Nucl. Med.

The labelling of blood cells in plasma with ¹¹¹In-tropolonate

Br. J. Radiol.

The development of a clinical protocol for the radiolabelling of mixed leucocytes with ^99mTc-hexamethylpropylene amine oxime

Nucl. Med. Commun.

Metabolic trapping as a principle of radiopharmaceutical design: some factors responsible for the biodistribution of [¹⁸F]2-deoxy-2-fluoro-d-glucose

J. Nucl. Med.

Efficient stereospecific synthsis of no-carrier-added 2-[¹⁸F]-fluoro-2-deoxy-d-glucose using aminopolyether supported nucleophilic substitution

J. Nucl. Med.

Neutrophil metabolic activity in localised pulmonary inflammation measured non-invasively by positron-emission tomography (PET)

Physiologist

Cited by (99)

Gamma camera imaging of cardiac implantable electronic devices infection

White Blood Cell Labeling With<sup>18</sup>F,<sup>64</sup>Cu, and<sup>89</sup>Zr

Achievement in active agent structures as a power tools in tumor angiogenesis imaging

<sup>18</sup>F-FDG labelling of hematopoietic stem cells: Dynamic study of bone marrow homing by PET–CT imaging and impact on cell functionality

Increased choline uptake in macrophages and prostate cancer cells does not allow for differentiation between benign and malignant prostate pathologies

Positron emission tomography and autoradiography of <sup>18</sup>F-fluorodeoxyglucose labeled islets with or without warm ischemic stress in portal transplanted rats

The use of 2-[18F]fluoro-2-deoxy-d-glucose as a potential in vitro agent for labelling human granulocytes for clinical studies by positron emission tomography

Abstract

Basic methods for the study of phagocytosis

Methods Enzymol.

Measurement of neutrophil function: phagocytosis, degranulation, the respiratory burst and bacterial killing

In vivo measurement of the local metabolic response to lung inflammation in rabbits by positron emission tomography (PET)

Clin. Sci.

Optimum conditions for radiolabelling human granulocytes and mixed leucocytes with 111In-tropolonate

Eur. J. Nucl. Med.

The labelling of blood cells in plasma with 111In-tropolonate

Br. J. Radiol.

The development of a clinical protocol for the radiolabelling of mixed leucocytes with 99mTc-hexamethylpropylene amine oxime

Nucl. Med. Commun.

Metabolic trapping as a principle of radiopharmaceutical design: some factors responsible for the biodistribution of [18F]2-deoxy-2-fluoro-d-glucose

J. Nucl. Med.

Efficient stereospecific synthsis of no-carrier-added 2-[18F]-fluoro-2-deoxy-d-glucose using aminopolyether supported nucleophilic substitution

J. Nucl. Med.

Neutrophil metabolic activity in localised pulmonary inflammation measured non-invasively by positron-emission tomography (PET)

Physiologist

The use of 2-[¹⁸F]fluoro-2-deoxy-d-glucose as a potential in vitro agent for labelling human granulocytes for clinical studies by positron emission tomography

Optimum conditions for radiolabelling human granulocytes and mixed leucocytes with ¹¹¹In-tropolonate

The labelling of blood cells in plasma with ¹¹¹In-tropolonate

The development of a clinical protocol for the radiolabelling of mixed leucocytes with ^99mTc-hexamethylpropylene amine oxime

Metabolic trapping as a principle of radiopharmaceutical design: some factors responsible for the biodistribution of [¹⁸F]2-deoxy-2-fluoro-d-glucose

Efficient stereospecific synthsis of no-carrier-added 2-[¹⁸F]-fluoro-2-deoxy-d-glucose using aminopolyether supported nucleophilic substitution