Plasma concentration of acetylcholine in young women
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Cited by (37)
Determination of non-neuronal acetylcholine in human peripheral blood mononuclear cells by use of hydrophilic interaction ultra-performance liquid chromatography-tandem mass spectrometry
2016, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life SciencesCitation Excerpt :Although the AChE mRNA levels also correlated with VAChT mRNA levels (Spearman r = 0.68, P < 0.01) (Fig. 4A–C), no such correlations were noted between plasma AChE activity and intracellular AChE activity (Spearman r = 0.12, P = 0.535) in volunteers (Fig. 4D). Kawashima detected and measured ACh concentrations in human plasma as about 0.4 ng/mL [8]. However, with our more sensitive UPLC–MS/MS detection method, we found that human plasma lacks ACh, regardless of our addition of adequate AChE inhibitor or not (data not shown).
Non-neuronal cholinergic system in regulation of immune function with a focus on α7 nAChRs
2015, International ImmunopharmacologyCitation Excerpt :Then in 1987, using a sensitive and specific radioimmunoassay (RIA) for ACh [26] and paraoxon, an instantaneously active AChE inhibitor, we detected 456 ± 53 pg/ml of ACh in the plasma of young women (n = 32) [27]. We then subsequently confirmed the presence of ACh in blood and plasma from various mammalian species, including humans (Table 1) [27–32]. The ACh levels in bovine blood were about 40 times higher than in human, and the levels in equine blood were about 10 times higher.
Critical roles of acetylcholine and the muscarinic and nicotinic acetylcholine receptors in the regulation of immune function
2012, Life SciencesCitation Excerpt :However, no detectable amount of ACh was found in either the PMNL fraction, which consists of mainly neutrophils, or the red cell fraction. The remaining portion of blood ACh appears to be present largely in plasma, because we previously found a plasma ACh level of 456 ± 53 (mean ± SEM) pg/ml (corresponding to 36% of the total blood ACh content) in 32 young women (Kawashima et al., 1987). The findings suggest that ACh in the blood originates primarily from lymphocytes.
Angiogenic activity of nicotinic acetylcholine receptors: Implications in tobacco-related vascular diseases
2009, Pharmacology and TherapeuticsExtraneuronal cholinergic system in lymphocytes
2000, Pharmacology and Therapeutics