The effect of temperature on the binding kinetics and equilibrium constants of monoclonal antibodies to cell surface antigens
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2022, Energy and BuildingsCitation Excerpt :Comfortable and partially warm environments increase S-IgE concentration in saliva, which may improve the respiratory tract mucosal immunity thereby improving the ability to resist parasite infection. Several studies have demonstrated that cold environments suppress cell- and antibody-mediated immunity, including inhibition of lymphokine release, reduction of leukocyte secretoryand antibody secretion, and protein mRNA gene coding disorder [29,30,78-85]. The mucosal immunity of the respiratory tract is regulated mainly by the autonomic nervous system.
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2021, Journal of Colloid and Interface ScienceCitation Excerpt :First, as outlined above, inhibiting uptake is typically performed by cooling the cells to 4 °C. Binding affinity is temperature dependant and the KD for some antibodies changes by more than an order between 4 °C and 37 °C [43]. This leads to significant differences in binding to cells at the different temperatures, thus making it difficult to determine if the differences in signal are due to binding or uptake.
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2020, Biophysical JournalCitation Excerpt :Febrile temperatures have been demonstrated to not only stiffen late stage IRBCs (15) but also enhance and accelerate the expression of adhesion ligands on the host membrane (16,17). Moreover, studies have revealed that temperature can modify antibody-antigen binding kinetics (18,19) and protein structure (20,21). It is hence likely that temperature variations during malaria infection can alter the binding kinetics of IRBC-receptor interactions and their protein structures.