Software noticeTwo-graph receiver operating characteristic (TG-ROC): a Microsoft-EXCEL template for the selection of cut-off values in diagnostic tests
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A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests
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Cited by (108)
Validation of laboratory developed serology assays for detection of IgG antibody to severe acute respiratory syndrome coronavirus 2 in the South African population
2022, Journal of Virological MethodsCitation Excerpt :In many instances a cut-off is determined using two or three standard deviations above and below the mean value however this has limitations as it assumes a normal distribution of test values in the target population. The application of a TG-ROC provides an alternative method for determining a cut-off (Greiner, 1995). In this validation data normalization was achieved by expressing OD values as a percentage of a known positive control included in each run.
Comparison of protectiveness of recombinant Babesia ovis apical membrane antigen 1 and B. ovis-infected cell line as vaccines against ovine babesiosis
2020, Ticks and Tick-borne DiseasesCitation Excerpt :Each plate consisted of four replicates for each of negative, positive and conjugate controls. The mean of the OD plus twice-standard deviation (mean OD + 2SD) was accepted as “cut-off” value for each plate (Greiner, 1995). Positive and negative threshold percentage positivity (PP) values were chosen arbitrarily to obtain the optimal sensitivity and specificity using a set of post infection serum samples as a known positive and a pool of pre-infected serum samples (n = 10) was use as a negative control.
Preoperative multiplex nomogram for prediction of high-risk nonorgan-confined upper-tract urothelial carcinoma
2019, Urologic Oncology: Seminars and Original InvestigationsMetaperiodate deglycosylation of Strongyloides venezuelensis larvae: Immunochemical characterization and antigen production for human strongyloidiasis diagnosis
2018, Acta TropicaCitation Excerpt :Two-way ANOVA with the antigens (SE and MP) and IgG subclasses factors, followed by the Šidák multiple comparison test, were applied to determine how the response/detection was affected by two factors. Receiver operating characteristic (ROC) and two-graph ROC (TG-ROC) curves were built to describe the tests and define the optimum point of each reaction condition (cut-off) (Greiner, 1995; Greiner et al., 1995). Tests were considered positive when OD > cut-off.
Missing values and inconclusive results in diagnostic studies – A scoping review of methods
2023, Statistical Methods in Medical Research
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