Elsevier

Brain Research

Volume 490, Issue 2, 26 June 1989, Pages 378-384
Brain Research

Adenosine antagonists delay hypoxia-induced depression of neuronal activity in hippocampal brain slice

https://doi.org/10.1016/0006-8993(89)90258-8Get rights and content

Abstract

Submerged rat hippocampal slices were exposed to hypoxic medium prepared with 95% N2/5% CO2. The population spikes recorded from CA1 cell layer were completely blocked within a range of 5–10 min. The adenosine antagonist theophylline (100 μM) delayed and partially prevented the hypoxia-induced depression. Increasing concentrations of the more potent adenosine antagonist 8-phenyltheophylline (8-PT; 0.1, 1, 10 μM) resulted in progressively less hypoxia-induced depression. The antidromically elicited afterpotentials recorded in the absence of synaptic transmission in low calcium, high magnesium medium were blocked within 8 min of hypoxia. Theophylline (100 μM) and 8-PT (10 μM) delayed to a similar extent the hypoxia-induced depression of the first afterpotential but did not prevent its complete depression.

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    The review of an earlier manuscript by Drs. John F. McElroy and Donal G. Sinex is appreciated. This work was supported by institutionally supported research funds of the Los Alamos National Laboratory under the auspices of the Department of Energy.

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