A procedure for eliminating interferences in the lowry method of protein determination
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2020, Journal of Experimental Marine Biology and EcologyCitation Excerpt :The Lowry method (Lowry et al., 1951) has been the most widely used colorimetric method for protein determination, but it is slow in spite of the research done to improve and simplify it (Markwell et al., 1978; Rutter, 1967). Ways to eliminate chemical side-reactions (Dulley and Grieve, 1975; Ji, 1973; Peterson, 1977; Rodríguez-Vico et al., 1989; Wang and Smith, 1975) and new, more accurate methods, based on unique dye-binding chemistry have been developed (Bradford, 1976; Smith et al., 1985). As examples of the changes in methodology, in the Rutter protein method, the sodium‑potassium tartrate concentration is reduced by 88% while in the Markwell et al. (1978) method, it is reduced from 1% to 0.16%.
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