Skip to main content

Advertisement

SpringerLink
Log in

Overexpression of MAC30 is associated with poor clinical outcome in human non-small-cell lung cancer

  • Research Article
  • Published:
Tumor Biology

Abstract

The aim of this study was to detect MAC30 expression in human non-small cell lung cancer (NSCLC) and to analyze its association with prognosis of NSCLC patients. Quantitative real-time RT-PCR was performed to examine the expression of MAC30 mRNA in 20 cases of NSCLC and corresponding non-tumor tissue samples. Immunohistochemistry was performed to detect the expression of MAC30 in 95 NSCLC tissues. We found that the expression levels of MAC30 mRNA in NSCLC tissues were significantly higher than those in corresponding non-tumor tissues. High-level MAC30 expression was correlated with poor tumor differentiation, TNM stage, and lymph node metastasis. Patients with high expression levels of MAC30 showed lower overall survival rate than those with low expression levels. Multivariate analysis showed that high MAC30 protein expression was an independent prognostic factor for NSCLC patients. Our study suggests that over-expression of MAC30 may play an important role in the progression of NSCLC and MAC30 expression may offer a valuable marker for predicting the outcome of patients with NSCLC.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, et al. Cancer statistics, 2008. CA Cancer J Clin. 2008;58:71–96. doi:10.3322/CA.2007.0010.

    Article  PubMed  Google Scholar 

  2. Kamangar F, Dores GM, Anderson WF. Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 2006;24:2137–50. doi:10.1200/JCO.2005.05.2308.

    Article  PubMed  Google Scholar 

  3. Murphy M, Pykett MJ, Harnish P, Zang KD, George DL. Identification and characterization of genes differentially expressed in meningiomas. Cell Growth Differ. 1993;4:715–22.

    PubMed  CAS  Google Scholar 

  4. Malhotra K, Luehrsen KR, Costello LL, Raich TJ, Sim K, Foltz L, et al. Identification of differentially expressed mRNAs in human fetal liver across gestation. Nucleic Acids Res. 1999;27:839–47.

    Article  PubMed  CAS  Google Scholar 

  5. Kayed H, Kleeff J, Ding J, Hammer J, Giese T, Zentgraf H, et al. Expression analysis of MAC30 in human pancreatic cancer and tumors of the gastrointestinal tract. Histol Histopathol. 2004;19:1021–31.

    PubMed  CAS  Google Scholar 

  6. Potapova O, Anisimov SV, Gorospe M, Dougherty RH, Gaarde WA, Boheler KR, et al. Targets of c-Jun NH(2)-terminal kinase 2-mediated tumor growth regulation revealed by serial analysis of gene expression. Cancer Res. 2002;62:3257–63.

    PubMed  CAS  Google Scholar 

  7. Sobin LH, Fleming ID. Union Internationale Contre le Cancer and the American Joint Committee on Cancer. TNM classification of malignant tumors, fifth edition. Cancer. 1997;80:1803–4.

    Article  PubMed  CAS  Google Scholar 

  8. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 2001;25:402–8.

    Article  PubMed  CAS  Google Scholar 

  9. Kelly K, Crowley J, Bunn Jr PA, Presant CA, Grevstad PK, Moinpour CM, et al. Randomized phase III trial of paclitaxel plus carboplatin versus vinorelbine plus cisplatin in the treatment of patients with advanced non-small-cell lung cancer: a Southwest Oncology Group trial. J Clin Oncol. 2001;19:3210–8.

    PubMed  CAS  Google Scholar 

  10. Hennessy BT, Hanrahan EO, Daly PA. Non-Hodgkin lymphoma: an update. Lancet Oncol. 2004;5:341–53.

    Article  PubMed  Google Scholar 

  11. Xiao M, Li H, Yang S, Huang Y, Jia S, Wang H, et al. Expression of MAC30 protein is related to survival and clinicopathological variables in breast cancer. J Surg Oncol. 2012. doi:10.1002/jso.23269.

  12. Zhao ZR, Zhang LJ, He XQ, Zhang ZY, Zhang F, Li F, et al. Significance of mRNA and protein expression of MAC30 in progression of colorectal cancer. Chemotherapy. 2011;57:394–401.

    Article  PubMed  CAS  Google Scholar 

  13. Yan BY, Wang DW, Zhu ZL, Yang YH, Wang MW, Cui DS, et al. Overexpression of MAC30 in the cytoplasm of oral squamous cell carcinoma predicts nodal metastasis and poor differentiation. Chemotherapy. 2010;56(6):424–8.

    Article  PubMed  CAS  Google Scholar 

  14. Zhang ZY, Zhao ZR, Adell G, Jarlsfelt I, Cui YX, Kayed H, et al. Expression of MAC30 in rectal cancers with or without preoperative radiotherapy. Oncology. 2006;71:259–65.

    Article  PubMed  CAS  Google Scholar 

  15. Moparthi SB, Arbman G, Wallin A, Kayed H, Kleeff J, Zentgraf H, et al. Expression of MAC30 protein is related to survival and biological variables in primary and metastatic colorectal cancers. Int J Oncol. 2007;30(1):91–5.

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by research grants from Project of Education Department of Heilongjiang Province (no. 12521z021 and 11521096), China

Conflicts of interest

None

Author information

Authors and Affiliations

  1. Department of Respiratory Medicine, The Second Affiliated Hospital of Harbin Medical University, No. 148, Baojian Rd, Nangang District, Harbin, 150086, Heilongjiang Province, China

    Kai-Yu Han, Fu-Zhen Lv & Jia-Ning Li

  2. Department of Head and Neck Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, 150086, Heilongjiang Province, China

    Xin Gu

  3. Department of Internal Medicine, The fourth hospital of Heilongjiang Province, Harbin, 150500, Heilongjiang Province, China

    Hao-Ran Wang

  4. Department of General Surgery, The fourth hospital of Heilongjiang Province, Harbin, 150500, Heilongjiang Province, China

    Dong Liu

Authors
  1. Kai-Yu Han
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xin Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hao-Ran Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dong Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fu-Zhen Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jia-Ning Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jia-Ning Li.

Additional information

Kai-Yu Han and Xin Gu contributed to this paper equally.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Han, KY., Gu, X., Wang, HR. et al. Overexpression of MAC30 is associated with poor clinical outcome in human non-small-cell lung cancer. Tumor Biol. 34, 821–825 (2013). https://doi.org/10.1007/s13277-012-0612-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13277-012-0612-z

Keywords

Search

Navigation