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The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFα and IL-1β after cerebral ischemia in rats

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  • Drug Efficacy and Safety
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Abstract

Previous studies have shown that Qing Kai Ling, a traditional Chinese medicine, was able to effectively prevent the inflammation from cerebral ischemia (Chen et al., 2002). The cholalic acid and hyodeoxycholalic acid (cholalic acid mixture) was major active components in Qing Kai Ling. To study the effects of cholalic acid mixture on the damage cascade of cerebral ischemia, rat model of focal cerebral ischemia was established by permanent occlusion of left middle cerebral artery. We found that the administration of cholalic acid mixture could reduce the ischemic infarct size after 24 h of ischemia, and cholalic acid mixture could be detected in cerebrospinal fluid after 2h of administration. We also found that the concentrations of tumor necrosis factor-alpha and interlukin-1beta in rat brain were significantly lower when compared to the untreated animals after 12 h and 24 h of ischemia. The concentrations of von Willebrand factor and neuron specific enolase in the plasma were remarkably decreased in cholalic acid mixture treated animals than in the untreated ones after 12h of ischemia. Our results suggested that cholalic acid mixture is able to decrease the expression of inflammation factors including tumor necrosis factor-alpha and interlukin-1beta after focal cerebral ischemia.

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Abbreviations

(TNF-α):

tumor necrosis factor-alpha

(IL-1β):

interlukin-1 beta

(NMDA):

N-methyl-D-aspartate

(ICAM-1):

intercellular adhensive molecular-1

(vWF):

von Willebrand factor

(NSE):

neuron-specific enolase

(QKL):

Qing Kai Ling

(MCAO):

middle cerebral artery occlusion

(ECA):

external carotid artery

(ICA):

internal carotid artery

(RIA):

Radioimmunoassay

(ip.):

intra-peritoneally

(BBB):

brain blood barrier

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Authors and Affiliations

  1. School of Preclinical Medicine, Beijing University of Chinese Medicine, 11 Bei San Huan Dong Road, Chaoyang District, Beijing, 100029, China

    Qian Hua, Xiao-lei Zhu, Peng-tao Li, Yuan Liu, Na Zhang, Ya Xu & Xu Jia

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  1. Qian Hua
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  2. Xiao-lei Zhu
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  3. Peng-tao Li
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  4. Yuan Liu
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  5. Na Zhang
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  6. Ya Xu
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  7. Xu Jia
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Correspondence to Peng-tao Li.

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These two authors contribute equally to the paper.

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Hua, Q., Zhu, Xl., Li, Pt. et al. The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFα and IL-1β after cerebral ischemia in rats. Arch. Pharm. Res. 32, 65–73 (2009). https://doi.org/10.1007/s12272-009-1119-z

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  • DOI: https://doi.org/10.1007/s12272-009-1119-z

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