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Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes

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Abstract

Purpose

The purpose of our study was to evaluate the degree of radiotoxicity to lymphocytes in thyroid cancer after iodine-131(I-131) therapy using γ-H2AX foci immunodetection.

Methods

This study focused on 15 patients who underwent I-131 therapy for differentiated thyroid cancer after surgery. All patients received 3.7 GBq of I-131. Venous blood samples were collected from each patient before therapy and 4 days thereafter. Lymphocytes were isolated from the blood samples and subjected to γ-H2AX immunofluorescence staining.

Results

The number (mean ± SD) of foci per lymphocyte nucleus was 0.41 ± 0.51 before and 6.19 ± 1.80 after radioiodine therapy, and this difference was statistically significant (P = 0.001 < 0.05). Absorbed doses estimated for the 15 patients were 0.77 ± 0.31 Gy applying standard line in vitro external radiation doses.

Conclusion

γ-H2AX foci immunodetection in lymphocytes may detect radiation-induced DNA damage associated with I-131 therapy for thyroid cancer, and may facilitate estimation of the radiation doses absorbed with this therapy.

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Acknowledgments

This study was supported in part by a Grant-in-Aid for scientific research from the Japan Society for the promotion of Science, Grant for Collaborative Research from Kanazawa Medical University (C2009-5,C2010-3), Grant for Project Research from High-Tech Research Center of Kanazawa Medical University (H2010-10, H2011-10), Assist KAKEN from Kanazawa Medical University and Hokkoku foundation for cancer research.

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Authors and Affiliations

  1. Department of Radiology, Kanazawa Medical University, Daigaku 1-1, Uchinada, Kahoku, Ishikawa, 920-0293, Japan

    Mariko Doai, Naoto Watanabe, Tomoko Takahashi, Mitsuru Taniguchi & Hisao Tonami

  2. Department of Biochemistry, Kanazawa Medical University, Daigaku 1-1, Uchinada, Kahoku, Ishikawa, 920-0293, Japan

    Kuniyoshi Iwabuchi

  3. Department of Nuclear Medicine, Kanazawa University, Takara-machi 13-1, Kanazawa, Ishikawa, 920-8641, Japan

    Daiki Kayano, Makoto Fukuoka & Seigo Kinuya

Authors
  1. Mariko Doai
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  2. Naoto Watanabe
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  3. Tomoko Takahashi
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  4. Mitsuru Taniguchi
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  5. Hisao Tonami
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  6. Kuniyoshi Iwabuchi
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  7. Daiki Kayano
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  8. Makoto Fukuoka
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  9. Seigo Kinuya
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Corresponding author

Correspondence to Mariko Doai.

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Doai, M., Watanabe, N., Takahashi, T. et al. Sensitive immunodetection of radiotoxicity after iodine-131 therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes. Ann Nucl Med 27, 233–238 (2013). https://doi.org/10.1007/s12149-012-0678-0

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  • DOI: https://doi.org/10.1007/s12149-012-0678-0

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