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SHARPIN overexpression induces tumorigenesis in human prostate cancer LNCaP, DU145 and PC-3 cells via NF-κB/ERK/Akt signaling pathway

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A Correction to this article was published on 31 July 2021

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Abstract

SHARPIN emerges higher expression in prostate cancerous tissues than in benign prostate hyperplasia by means of immunohistochemistry in our previous study. In this work, we performed the gain of function assay and find that overexpression of SHARPIN in LNCaP, DU145 and PC-3 cells promoted cell proliferation, invasiveness and reduced apoptosis. Furthermore, SHARPIN overexpression displayed elevated Bcl-2 and Survivin expression and reduced levels of Bax, cleaved caspase-3. Meanwhile, entropic expression of SHARPIN increased the levels of phosphorylated p65, IkBα, ERK and Akt, were selectively increased in these cells. Collectively, our study unraveled the ability of SHARPIN overexpression to induce tumorigenesis of prostate cancer cells through the NF-kB/ERK/Akt pathway and transformation of apoptosis-associated proteins.

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Acknowledgments

This research was supported by Grants from the National Natural Science Foundation of China [No. 81272807, 81472382], the National Natural Science Foundation of China for Young Scientists Grant [No. 81101947], Science and Technology Development Program of Guangdong Province [No. 2013B021800107].

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Authors and Affiliations

  1. Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People’s Republic of China

    Jin Li, Yiming Lai, Ganping Wang, Xianju Chen, Jieqing Chen, Yongsheng Yu, Simin Zhang, Yiming Zhang, Hai Huang & Zhenghui Guo

  2. The Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, People’s Republic of China

    Jin Li, Yiming Lai, Yi Cao, Tao Du, Lexiang Zeng, Ganping Wang, Xianju Chen, Jieqing Chen, Yongsheng Yu, Simin Zhang, Yiming Zhang, Hai Huang & Zhenghui Guo

  3. Department of Emergency, SunYat-sen Memorial Hospital, SunYat-sen University, Guangzhou, People’s Republic of China

    Yi Cao

  4. Prenatal Diagnosis Center, SunYat-sen Memorial Hospital, SunYat-sen University, Guangzhou, People’s Republic of China

    Tao Du

  5. Department of Pediatric Surgery, SunYat-sen Memorial Hospital, SunYat-sen University, Guangzhou, People’s Republic of China

    Lexiang Zeng

Authors
  1. Jin Li
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  2. Yiming Lai
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  7. Xianju Chen
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  9. Yongsheng Yu
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  10. Simin Zhang
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  11. Yiming Zhang
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  12. Hai Huang
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  13. Zhenghui Guo
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Corresponding authors

Correspondence to Hai Huang or Zhenghui Guo.

Additional information

Jin Li, Yiming Lai and Yi Cao have contributed equally to this work.

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Li, J., Lai, Y., Cao, Y. et al. SHARPIN overexpression induces tumorigenesis in human prostate cancer LNCaP, DU145 and PC-3 cells via NF-κB/ERK/Akt signaling pathway. Med Oncol 32, 1 (2015). https://doi.org/10.1007/s12032-014-0444-3

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  • DOI: https://doi.org/10.1007/s12032-014-0444-3

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