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Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of 44Sc-DOTA-Puromycin

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Abstract

Purpose

The purpose of this study was to investigate whether 44Sc-labeled puromycin can be utilized for imaging of protein synthesis in vivo.

Methods

For micro-positron emission tomographic (μPET) studies, 20–25 MBq of [44Sc]-DOTA-puromycin was administered to tumor-bearing rats, and animals were scanned for 1 h dynamically. Results were further validated by dissecting organs and tissues of the animals after the measurement and in vitro blocking experiments using puromycin or cycloheximide to block protein synthesis.

Results

μPET images of tumor-bearing rats showed significant tumor uptake of [44Sc]-DOTA-puromycin and a clear-cut tumor visualization. In both blocking experiments, cellular uptake of [44Sc]-DOTA-puromycin ([44Sc]-DOTA-Pur) could be suppressed by blocking protein synthesis.

Conclusions

We report for the first time successful μPET imaging with 44Sc obtained from a 44Ti/44Sc generator, as well as noninvasive μPET imaging of ribosomal activity, respectively protein synthesis, with a puromycin-based radiopharmaceutical and the direct correlation between cellular uptake of [44Sc]-DOTA-Pur and protein synthesis.

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Acknowledgments

This project was subsidized by the Ministry of Education, Youth and Sports of the Czech Republic: Project NPV II 2B06165.

We thank Dr. Nicole Bausbacher, Department of Nuclear Medicine, University Medicine, Mainz, Germany, and Barbara Biesalski for outstanding support on μPET imaging.

We thank Dr. Matthias Miederer, Department of Nuclear Medicine, University Medicine Mainz, Germany, for his support in fulfilling the requirements of the national animal protection laws.

Conflicts of interest

None.

Author information

Authors and Affiliations

  1. Nuclear Physics Institute, Department of Radiopharmaceuticals, Academy of Science of the Czech Republic, Husinec-Rez 289, 25068, Husinec-Rez, Czech Republic

    Sebastian Eigner, Denis R. Beckford Vera, Markus Piel, Ondrej Lebeda & Katerina Eigner Henke

  2. Institute for Nuclear Chemistry, Johannes Gutenberg-University, Mainz, Germany

    Marco Fellner, Natalia S. Loktionova, Frank Rösch & Tobias L. Roß

Authors
  1. Sebastian Eigner
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  2. Denis R. Beckford Vera
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  3. Marco Fellner
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  4. Natalia S. Loktionova
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  5. Markus Piel
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  6. Ondrej Lebeda
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  7. Frank Rösch
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  8. Tobias L. Roß
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  9. Katerina Eigner Henke
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Corresponding author

Correspondence to Sebastian Eigner.

Additional information

This study forms a part of the dissertations of S. Eigner and D. R. Beckford Vera.

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Eigner, S., Vera, D.R.B., Fellner, M. et al. Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of 44Sc-DOTA-Puromycin. Mol Imaging Biol 15, 79–86 (2013). https://doi.org/10.1007/s11307-012-0561-3

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  • DOI: https://doi.org/10.1007/s11307-012-0561-3

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