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Uptake of a Fluorescent Deoxyglucose Analog (2-NBDG) in Tumor Cells

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Abstract

Purpose

A new fluorescent analog of d-glucose was recently developed by [Yoshioka K, Takahashi H, Homma T, Sato M, Ki Bong O, Nemoto Y, Matsuoka H (1996) A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli. Biochim Biophys Acta 1289:5–9] and shown to be transported into normal cells. The purpose of this preliminary study was to assess the use of this fluorescent 2-deoxyglucose analog, 2-[N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG), as a sensitive probe for monitoring glucose uptake into malignant tumor cells.

Procedures

MCF-7 breast cancer epithelial cells were grown and plated on coverslips for analysis of 2-NBDG uptake via fluorescence imaging microscopy.

Results

Steady‐state fluorescence analysis of 2-NBDG uptake displayed rapid uptake for the first one to five minutes, then slowed, reaching an apparent maximum uptake near 20–30 minutes. Addition of 5 mM d-glucose to the media markedly reduced 2-NBDG uptake. Uptake of 2-NBDG in nonmalignant epithelial cells (M-1 epithelial cells) was slow, averaging less than 20% of that observed for tumorigenic cells, the MCF-7 breast cancer cells and the HepG2 liver cancer cell line.

Conclusions

The preliminary data clearly demonstrate a rapid uptake of 2-NBDG into tumor cells that can be monitored by fluorescence imaging analysis. The uptake displays saturation and competition with d-glucose, all properties expected for 2-NBDG uptake and retention in cancer cells. Additional studies, including comparisons among other malignant cell lines and control cells, will be needed to fully characterize the kinetic properties of 2-NBDG uptake and the potential use of this 2-DG analog as a probe for glucose uptake in malignant cells.

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Abbreviations

2-DG:

2-deoxyglucose

2-NBDG:

2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose

FDG:

2-deoxy-2-[F-18]fluoro-D-glucose

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Acknowledgment

This work was partially support by 3Gen LLC, Sugar Land, TX.

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Authors and Affiliations

  1. Department of Integrative Biology and Pharmacology, University of Texas Health Science Center, 6431 Fannin, Houston, TX, 77030, USA

    Roger G. O’Neil, Ling Wu & Nizar Mullani

Authors
  1. Roger G. O’Neil
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  2. Ling Wu
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  3. Nizar Mullani
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Correspondence to Roger G. O’Neil.

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O’Neil, R.G., Wu, L. & Mullani, N. Uptake of a Fluorescent Deoxyglucose Analog (2-NBDG) in Tumor Cells. Mol Imaging Biol 7, 388–392 (2005). https://doi.org/10.1007/s11307-005-0011-6

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  • DOI: https://doi.org/10.1007/s11307-005-0011-6

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