Abstract
A automated synthesis of symmetric integrin αvβ3-targeted radiotracer [18F]FP-PEG3-β-Glu-RGD2 was carried out by multi-step procedure on the modified PET-MF-2V-IT-I synthesizer. Firstly, the prosthetic group of 4-nitrophenyl 2-[18F]fluoropropionate ([18F]NFP) was automated synthesized by a convenient three-step, one-pot procedure. Secondly, [18F]FP-PEG3-β-Glu-RGD2 was synthesized by coupling [18F]NFP with the symmetric RGD-peptide (PEG3-β-Glu-RGD2) and purified by a solid-phase extraction cartridge. The radiochemical yields of [18F]NFP were 35 ± 5 % (n = 10, decay-corrected) based on [18F]fluoride in 80 min. [18F]FP-PEG3-β-Glu-RGD2 was obtained with yield 40 ± 10 % (n = 5, decay-corrected) from [18F]NFP within 20 min. The radiochemical purity of [18F]FP-PEG3-β-Glu-RGD2 was greater than 98 %.
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Acknowledgments
We thank Zhaobao Ye and Kening Wu for assistance with this work. This work was partly supported by the National High Technology Research and Development Program of China (863 Program, No. 2008AA02Z430), the National Natural Science Foundation (No. 30970856, No. 81371584, No. 81101076), the International Atomic Energy Agency (IAEA) Research Contract (No. 15245), the Science Technology Foundation of Guangdong Province (No. 2010B031600054), and Sun Yat-Sen University (No. 80000-3126132).
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Hu, KZ., Wang, H., Tang, G. et al. Automated synthesis of symmetric integrin αvβ3-targeted radiotracer [18F]FP-PEG3-β-Glu-RGD2 . J Radioanal Nucl Chem 299, 271–276 (2014). https://doi.org/10.1007/s10967-013-2736-z
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DOI: https://doi.org/10.1007/s10967-013-2736-z