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Increased uptake of folate conjugates by activated macrophages in experimental hyperlipemia

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Abstract

In the pathogenesis of atherosclerosis, macrophages become activated and play a crucial role in plaque formation. Activated synovial macrophages have recently been shown to express receptors for folic acid. We have determined whether activated macrophages also over-express folate receptor (FR) in atherosclerosis. Most normal cells express little or no FR, and, if FR is present on activated macrophages, folate-linked compounds and drugs could be selectively targeted to those cells that do express FR. To evaluate the FR on macrophages of atherosclerotic animals, golden Syrian hamsters were maintained on a hyperlipidemic diet until extensive vascular lesions had developed. Uptake of folic acid conjugated to fluorescent tags was then examined in tissue fragments from lesion-prone areas, and peritoneal activated macrophages were harvested from the same animals. Spectrofluorimetric and fluorescence microscopic analyses showed a significantly greater uptake of folate-conjugates by peritoneal macrophages of hyperlipidemic hamsters compared with those of hamsters fed a normal or folate-deficient diet. Systemically administered folate-fluorescent conjugates were found to accumulate as bright spots in protrusions of atherosclerotic plaques populated by macrophages, whereas a low level of fluorescence was detected uniformly dispersed across the lesion. The uptake of the folate conjugate by U937 macrophage cells grown in a high-lipid culture medium was significantly higher than in controls. Our data thus indicate that hyperlipidemic conditions induce an increased uptake of folate attributable to the over-expression of FRs on activated macrophages. This increase in FR expression can be exploited to deliver folate-linked compounds selectively to atherosclerotic lesions.

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Acknowledgements

The authors are grateful to Emanuela Constantinescu (biochemistry), Daniela Rogoz (spectrophotofluorimetry), Emanuel Dragan, Nae Safta (animal housing), and Mariana Pascu (biochemistry).

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Correspondence to Felicia Antohe.

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This work was supported by a grant from the Romanian Academy and Ministry of Education, Research and Technology, Bucharest, Romania, and partially by a grant from Endocyte and the Indiana 21st Century Fund.

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Antohe, F., Radulescu, L., Puchianu, E. et al. Increased uptake of folate conjugates by activated macrophages in experimental hyperlipemia. Cell Tissue Res 320, 277–285 (2005). https://doi.org/10.1007/s00441-004-1071-7

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  • DOI: https://doi.org/10.1007/s00441-004-1071-7

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