Abstract
ABCC2 is a member of the multidrug resistance protein subfamily localized exclusively to the apical membrane domain of polarized cells, such as hepatocytes, renal proximal tubule epithelia, and intestinal epithelia. This localization supports the function of ABCC2 in the terminal excretion and detoxification of endogenous and xenobiotic organic anions, particularly in the unidirectional efflux of substances conjugated with glutathione, glucuronate, or sulfate, as exemplified by leukotriene C4, bilirubin glucuronosides, and some steroid sulfates. The hepatic ABCC2 pump contributes to the driving forces of bile flow. Acquired or hereditary deficiency of ABCC2, the latter known as Dubin–Johnson syndrome in humans, causes an increased concentration of bilirubin glucuronosides in blood because of their efflux from hepatocytes via the basolateral ABCC3, which compensates for the deficiency in ABCC2-mediated apical efflux. In this article we provide an overview on the molecular characteristics of ABCC2 and its expression in various tissues and species. We discuss the transcriptional and posttranscriptional regulation of ABCC2 and review approaches to the functional analysis providing information on its substrate specificity. A comprehensive list of sequence variants in the human ABCC2 gene summarizes predicted and proven functional consequences, including variants leading to Dubin–Johnson syndrome.
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Introduction
The multidrug resistance protein 2 (ABCC2) was the second member of the subfamily of MRP efflux pumps to be cloned from rat and human tissues [13, 60, 114, 133, 165]. The function of rat Abcc2 was recognized long before its cloning by studies on the hepatobiliary elimination of organic anions in normal and transport-deficient mutant rats [54, 55, 64, 131, 163]. The loss of ATP-dependent transport across the hepatocyte canalicular membrane was identified in these mutant rats using inside-out membrane vesicles and various glutathione (GSH) S-conjugates as substrates, notably leukotriene C4 (LTC4) [57]. Thus, the difference between canalicular membrane vesicles from normal and transport-deficient mutant rats with respect to ATP-dependent transport of various anionic substrates has provided important insight into the substrate specificity of the conjugate export pump Abcc2. Moreover, the similar substrate spectrum of Abcc2 and ABCC1 [89] has supported the identification of substrates for ABCC1, which had been cloned by Cole et al. [21], 2 years before the identification of the first transport substrates of ABCC1 [105, 120]. The link between the ATP-dependent transport of LTC4 and ABCC1 was made primarily by photoaffinity labeling using LTC4 as a photolabile high-affinity substrate which served to label mouse Abcc1 [106] and human ABCC1 ([66, 105]; reviewed by Jedlitschky and Keppler [65]). This photoaffinity labeling approach [33, 105, 106] had indicated a 190-kDa integral membrane glycoprotein with properties similar to ABCC1 [106].
The localization of ABCC2 to the apical membrane of various polarized cells involved in the secretion of conjugated endogenous and xenobiotic substances favors the function of this efflux pump in the terminal phase of detoxification [83, 89]. Due to this substrate specificity, ABCC2, rather than ABCB1 or ABCG2, is the optimal pump for the elimination of conjugates of various toxins and carcinogens with GSH, glucuronate, or sulfate from hepatocytes into bile, or from kidney proximal tubules into urine, or from intestinal epithelial cells into the intestinal lumen. Particularly in nonpolarized cell types, additional members of the ABCC subfamily, such as ABCC1, may have this function in terminal detoxification.
ABCC2 plays a decisive role in the elimination of bilirubin glucuronosides from hepatocytes into bile. Accordingly, the absence of functional ABCC2 from the canalicular membrane [72, 82] causes conjugated hyperbilirubinemia, as observed in the hereditary disorder described by Dubin and Johnson [31]. Today, many sequence variants in the ABCC2 gene have been identified, as described below, but only some of them cause Dubin–Johnson syndrome. The hereditary deficiency of ABCC2 in humans, or of Abcc2 in the mutant rats mentioned above, and the recently generated Abcc2 knock-out mouse strains [18, 121, 177] illustrate the key function of this apical efflux pump in the elimination of anionic conjugates from the body. It should be noted, however, that this loss of ABCC2 function is usually well tolerated and compensated by the upregulation of other membrane transporters, particularly of ABCC3 in the basolateral membrane of hepatocytes [30, 82, 91].
Functional characterization and substrate specificity of ABCC2
Insight into the substrate specificity of human or rodent ABCC2/Abcc2 may be obtained on several experimental levels. A relatively simple indication whether a compound is transported by rat Abcc2 is given by the comparative elimination of a compound into the bile of normal and Abcc2-deficient mutant rats [55, 64, 131]; however, intracellular metabolism and the presence of alternative efflux pumps in the canalicular membrane may obscure the results. Kinetic constants for rat Abcc2 may be obtained by comparative studies with inside-out hepatocyte canalicular membrane vesicles isolated from normal and Abcc2-deficient liver [57, 126, 127, 187], as detailed in Table 1. Clearly, the most informative data can be obtained with inside-out membrane vesicles containing recombinant ABCC2 from human or other species expressed in cell lines such as human embryonic kidney cell line 293 [24], Madin–Darby canine kidney cell line (MDCKII) [24, 32], or Sf9 insect cells [171]. K m values given for various substrates have been obtained with these inside-out membrane vesicles as summarized in Table 1. ABCC2 purified to homogeneity has also been analyzed with respect to the ATP-dependent transport of LTC4 and ATPase activity [43, 44]. The ATPase activity of ABCC2 has been studied in detail in membrane vesicles [4]. Although this assay may provide valuable information, it cannot substitute for transport measurements using inside-out membrane vesicles, which allow for the determination of K m values and for kinetic constants of inhibitors of transport across membranes.
More recently, double- and multiple-transfected polarized cells have been developed, which stably express an uptake transporter of the organic anion transporting polypeptide family in the basolateral membrane and ABCC2 in the apical membrane [25, 81, 95, 149] (Fig. 1). With this in vitro system, functional analyses and identification of substrates for ABCC2 have become possible in a medium- to high-throughput format. However, K m values cannot be obtained by the use of such double-transfected cell lines.
The substrate specificity of ABCC2 is broad and comprises many organic anions, with the highest affinity for glucuronate and GSH conjugates of lipophilic substances, as exemplified by LTC4, which is an arachidonate derivative and a GSH conjugate (Table 1, reviewed by Jedlitschky and Keppler [65] and König et al. [91]). In addition, many anionic substances without anionic conjugate residues have been identified as substrates, such as methotrexate and bromosulfophthalein (Table 1). Reduced and oxidized GSH are additional substrates. Interestingly, Abcc2-deficient mutant rats cannot excrete GSH into their bile [131, 135]. It remains controversial whether this is due to low affinity transport of GSH by Abcc2 or due to the coefflux of GSH with additional compounds in a process well established for ABCC1 [110] and for ABCC4 [140, 141]. The differences in the substrate specificity between ABCC2 and ABCC1 are limited and often detectable only by the determination of kinetic constants [65, 89].
Molecular characterization of ABCC2
Abcc2 cDNA was initially identified and cloned as a fragment from rat liver, using degenerate oligonucleotides complementary to human ABCC1 [21] and Leishmania ltpgpA [114]. This Abcc2 cDNA fragment was amplified from normal rat liver, but not from the liver of mutant rats that were deficient in the hepatobiliary excretion of anionic conjugates [114]. The subsequent cloning and analysis of the full-length rat Abcc2 cDNA identified Abcc2 [Mrp2; formerly described as cMrp and canalicular multispecific organic anion transporter (cMoat)] as an ABCC1-related protein localized in the canalicular membrane of hepatocytes [13, 133] (Fig. 1). At present, the full-length sequences from six mammalian species, including the orthologs from human, rhesus monkey, rat, rabbit, mouse, dog [13, 22, 37, 133, 165, 172], and from three other vertebrates, are known (Table 2). ABCC2-related sequences are also present in many other organisms, including Caenorhabditis elegans [11] and Arabidopsis thaliana [139]. Within the human ABCC subfamily, ABCC2 has the highest degree of amino acid sequence identity with ABCC1 (50%) and the lowest with ABCC11 and cystic fibrosis transmembrane conductance regulator (Table 2). ABCC2 is structurally and functionally very distinct from MDR1 P-glycoprotein (ABCB1), which belongs to a different subfamily of ABC transporters with which ABCC2 shares only about 26% amino acid sequence identity. The human ABCC2 gene is located on chromosome 10q24 [165], spans about 65 kilobase pairs, and consists of 32 exons with a high proportion of class 0 introns [168, 170].
The predicted topology of the subfamily of ABCC/MRP efflux pumps is related to most of the other human ABC transporters and comprises two polytopic membrane-spanning domains, each followed by a nucleotide-binding domain [45, 96]. However, ABCC2, together with ABCC1, ABCC3, ABCC6, and ABCC10, is unique in having an additional amino-terminal polytopic membrane-spanning domain (Fig. 2) that may be required for proper function and localization [34, 39, 183]. The extracellular location of the amino terminus was first predicted for rat Abcc2 by computational analysis [13] and subsequently proven experimentally [24]. Different algorithms, which have been used to calculate the probability of an amino acid sequence to be a transmembrane-spanning helix in a polytopic membrane protein, predicted only four transmembrane-spanning helices between the first and second nucleotide-binding domain of ABCC2 [91] (Fig. 2). This is in contrast to the established six transmembrane-spanning helices in the corresponding part of ABCC1 [21].
Localization of ABCC2 in polarized cells and tissues
The ABCC2 protein was, for the first time, localized in the apical (canalicular) membrane of rat [13] and human [82, 134] hepatocytes and, therefore, initially termed canalicular MRP (cMRP) [13] or cMOAT [133, 134, 165]. The subsequent identification of Abcc2 in the apical membrane of proximal tubule epithelial cells of rat kidney was the first demonstration of an extrahepatic localization of this conjugate efflux pump [150]. Besides human kidney [151], other polarized human tissues with ABCC2 being present in the apical membrane of the polarized cells include the small intestine [38, 147], colon [91], gallbladder [144], bronchi [91, 147], and placenta [159] (Fig. 1). Many of these polarized cell types express additional ABCC/MRP efflux pumps that are basolaterally localized, as exemplified in human hepatocytes where ABCC3 [90, 94], ABCC4 [140], and ABCC6 [6, 91] were identified in the sinusoidal membrane. In contrast to these polarized tissues, the ABCC2 protein is apparently not present in the human blood–testis barrier [5, 147] and in the blood–brain barrier in the human cortex [125, 147, 188]. However, ABCC2 was identified in brain capillaries of hippocampus specimens from patients with temporal lobe epilepsy [2] and in the rat blood–brain barrier after pilocarpine-induced epileptic seizures [52]. Moreover, some studies described Abcc2 in brain capillaries of normal rats [115, 176]. Various human tissues do not express the ABCC2 protein in detectable amounts, such as the skin, exocrine pancreas, female reproductive system, lymphatic system, cardiovascular system, and connective tissue [147].
In addition to normal human tissues, the ABCC2 protein is also present in a number of human malignant tumors, as evidenced by immunostaining of clinical specimens from renal clear-cell [147, 151], hepatocellular [123, 190], ovarian [3, 147], and colorectal carcinomas [49, 147]. Using tissue microarrays, ABCC2 was detected in lung, breast, and gastric carcinomas [147]. In contrast, ABCC2 protein expression was negligible or absent in primary testicular tumors [5], pancreatic adenocarcinomas [92], and gliomas [12]. Because of its ability to confer resistance to a wide variety of anticancer drugs [17, 24, 53, 78], ABCC2 may be of clinical relevance by contributing to the multidrug resistance phenotype of several solid malignant human tumors.
Several cell lines endogenously expressing ABCC2 have been used to study ABCC2 function in intact cells. Some rat and human hepatoma cells acquire a hepatocyte-like polarity after several days in culture and form apical vacuoles or bile canaliculus-like structures between adjacent cells [14, 122]. Secretion of fluorescent ABCC2 substrates into these apical vacuoles can be followed by live cell video microscopy [14, 91, 122]. Caco-2 cells, which are derived from a human colon carcinoma and also endogenously express ABCC2, gain an epithelial polarity with ABCC2 being present in the apical membrane when grown on certain filter membranes [9, 182]. Polarized MDCK (or rather MDCKII) cells expressing recombinant human ABCC2 in their apical membrane are better defined as cell systems than Caco-2 cells to study the ABCC2-mediated efflux of substances from cells [24, 32]. A major advancement came with the development of MDCK cells coexpressing one [25, 149] or more [95] human organic anion uptake transporters, localized in the basolateral membrane, together with the human apical efflux pump ABCC2 (Fig. 1). These double or quadruple transfectants are useful to study the vectorial transport of substances that undergo hepatobiliary elimination in humans without the need for studies with inside-out membrane vesicles containing the ABCC2 protein [25, 81, 95, 108, 109, 149, 157].
ABCC2 is exclusively localized in the apical membrane of polarized cells. A cell type-dependent localization, as detected for ABCC4, which is present in the basolateral membrane of human hepatocytes [140] and in the apical membrane of kidney proximal tubule epithelial cells [174], has not been observed for ABCC2. Under certain experimental conditions, ABCC2 may be redistributed to the basolateral membrane, e.g., in rat hepatocyte couplets shortly after their isolation [142] and in human HepG2 cells after protein kinase C activation [100].
The molecular mechanisms by which ABCC2 is targeted to the apical membrane are incompletely understood. Some proteins have been reported to interact with ABCC2 in vitro. Radixin, a member of the ezrin/radixin/moesin family cross-linking actin with several integral membrane proteins [10], is apparently required for the proper apical localization of Abcc2 because radixin knock-out mice exhibit conjugated hyperbilirubinemia and a selective loss of the Abcc2 protein from the hepatocyte canalicular membrane [84]. Canalicular localization of human ABCC2 may similarly be dependent on radixin interaction [88]. Other proteins interacting with ABCC2 in vitro belong to the PSD95/Dlg/ZO-1 (PDZ) family and bind to C termini with the conserved sequence T/S–X–Φ, with X being any amino acid and Φ a hydrophobic amino acid. Interaction of ABCC2 with PDZK1 and other PDZ proteins has been described [48, 86] and most of the known ABCC2 orthologs (Table 2) have a consensus PDZ-binding motif at their C termini. Interestingly, PDZK1 knock-out mice have a proper apical localization of Abcc2 in kidney proximal tubule epithelial cells, indicating that the interaction of Abcc2 with PDZK1 is either not required in vivo or that other PDZ proteins are able to compensate [87]. Whereas one report proposed that the PDZ-binding motif is the apical targeting signal of ABCC2 [46], this was not confirmed by all other studies [34, 93, 124, 183]. These latter studies indicate that the apical targeting motif of ABCC2 is not a distinct, narrowly defined amino acid sequence, but rather appears to be composed of several motifs within different parts of ABCC2 that come together only in the intact protein.
Transcriptional and posttranscriptional regulation of ABCC2
ABCC2 is not only constitutively expressed, but its expression is also regulated on the transcriptional and posttranscriptional level in response to many endogenous and xenobiotic substances and to different disease states. Transcriptional regulation may result from changes in the intracellular concentrations of bile acids and of a number of lipophilic compounds that are ligands for nuclear hormone receptors. Additional posttranscriptional mechanisms may allow for a short-term regulation of ABCC2. ABCC2 regulation is often accompanied by a coordinate regulation of other bile salt transporters and of conjugating enzymes. These processes are described in detail by Gerk and Vore [40], Kullak-Ublick et al. [102], and van de Water et al. [175].
Cloning and characterization of the rat Abcc2 and the human ABCC2 promoters identified several consensus binding sites for both liver-specific and ubiquitous transcription factors [74, 160, 164]. The region between nucleotides −517 and −197 in front of the translation initiation codon is important for human ABCC2 expression [160], with the transcriptional start-site being at nucleotide −247. In addition, a hormone response element in the rat Abcc2 promoter (ER-8) was identified, which is bound by heterodimers of the retinoid X receptor α (RXRα, NR2B1 [129]) with the ligand-activated transcription factors farnesoid X receptor (FXR, NR1H4), pregnane X receptor (PXR, NR1I2), or constitutive androstane receptor (CAR, NR1I3) [73]. These nuclear receptors are, for example, activated by bile acids via FXR [132] by various xenobiotics such as the antibiotic rifampicin, the synthetic glucocorticoid dexamethasone, and pregnenolone 16α-carbonitrile via PXR [8, 41, 85], or by phenobarbital via CAR [161]. Knowledge of the presence of the hormone response element ER-8 in the rat Abcc2 promoter [73] may now explain studies which describe the induction of Abcc2 mRNA and Abcc2 protein in primary cultures of rat hepatocytes by a number of xenobiotics, including the carcinogen 2-acetylaminofluorene, the anticancer drug cisplatin, the antifungal agent clotrimazole, the antibiotic cycloheximide, dexamethasone, the chemopreventive agents oltipraz and sulforaphane, phenobarbital, and pregnenolone 16α-carbonitrile [23, 73, 75, 98, 137]. Additional factors are probably involved in ABCC2/Abcc2 regulation in vivo because phenobarbital or oltipraz treatment of rats did not increase Abcc2 mRNA expression [23, 40, 42, 68]. In contrast, the feeding of mice with several compounds, e.g., the bile acids ursodeoxycholic acid and cholic acid [36], or the herbicide 2,4,5-trichlorophenoxyacetic acid [184], induced Abcc2 mRNA and Abcc2 protein expression, which may prevent the accumulation of potentially toxic bile acid conjugates or xenobiotics. Similarly, ABCC2 induction was detected in the liver of nonhuman primates [76] and in the intestines of humans [38] after treatment with the PXR ligand rifampicin.
ABCC2/Abcc2 regulation in disease states can also, at least in part, result from mediation via transcription factors. Down-regulation of Abcc2 mRNA and Abcc2 protein in rat liver after experimental cholestasis induced either by bile duct ligation or endotoxin treatment [99, 169, 178] may be explained by an upregulation of the inflammatory cytokine interleukin-1β, which in turn down-regulates the heterodimeric retinoic acid receptor α/RXRα, leading to down-regulation of Abcc2 promoter activity [27]. Interestingly, Abcc2 expression in the kidney is preserved or up-regulated during cholestasis [27, 103]. In humans, ABCC2 mRNA levels were reduced in livers from patients with primary sclerosing cholangitis [130] or with obstructive cholestasis [153]. Obstructive cholestasis leads to reduced ABCC2 protein levels in the intestine without an effect on ABCC2 mRNA expression [28].
Besides this transcriptional regulation, several studies describe the posttranscriptional regulation of ABCC2/Abcc2. One mechanism is the modulation of the amount of the conjugate efflux pump in the apical membrane by recruitment of transporter molecules from intracellular pools or their retrieval into these pools. Insertion of Abcc2 into the canalicular membrane of rat hepatocytes may depend on the lipid kinase phosphoinositide 3-kinase [116], on protein kinase C [7], or on cyclic AMP [142]. Retrieval of rat Abcc2 transporter molecules from the canalicular membrane into the hepatocyte has been detected under different cholestatic conditions [119, 136, 169], after phalloidin treatment [143], after cytokine stimulation [29, 99], and under hyperosmolar conditions [29, 97]. Under these conditions, immunostaining of Abcc2 is no longer confined to the canalicular membrane, but appears “fuzzy,” which is interpreted as an accumulation of transporter molecules within the hepatocyte. A comparable fuzzy immunostaining, indicative of endocytic retrieval of transporter molecules, has been described in human liver diseases, e.g., inflammation-induced icteric cholestasis [189], obstructive cholestasis [153, 186], and advanced stages of primary biliary cirrhosis [88, 101].
The amount of transporter molecules in the hepatocyte canalicular membrane can also be regulated by a change in their synthesis or degradation rate. The physiological half-life of rat Abcc2 in the hepatocyte canalicular membrane is about 27 h [69]. In normal rat liver, the synthesis and degradation rates result in Abcc2 protein levels that appear to be similar within a lobule [136]. During cholestasis, however, Abcc2 in the periportal hepatocytes is more rapidly degraded than under normal conditions, leading to the observation that Abcc2 is concentrated near the central (perivenous) area of the liver lobule [136]. Decreased Abcc2 protein levels, but unaltered Abcc2 mRNA levels, were also detected in rats treated with ethinylestradiol [169] and in pregnant rats in comparison with control rats [15]. Clofibrate, which is a ligand for the peroxisome proliferator-activated receptor α (PPARα, NR1C1 [59]), also decreased rat Abcc2 protein levels by posttranscriptional mechanisms [68].
Sequence variants of ABCC2 and the hereditary deficiency of ABCC2 in human Dubin–Johnson syndrome and in animal models
More than 200 naturally occurring sequence variants have been identified in the exons, introns, and the 5′- and 3′-flanking regions of the human ABCC2 gene. Many of these sequence variants are single nucleotide changes in the introns or in the exons without leading to amino acid changes, and are, therefore, probably without functional consequences [62, 63, 117, 146, 162, 179] (single nucleotide polymorphism database, http://www.ncbi.nlm.nih.gov/SNP). Sequence variants that result in the absence of a functionally active ABCC2 protein from the canalicular membrane of hepatocytes are the molecular basis of Dubin–Johnson syndrome in humans [72, 82, 134, 170]. This syndrome is an autosomal, recessively inherited disorder characterized by conjugated hyperbilirubinemia (i.e., increased concentration of bilirubin glucuronosides in blood) and deposition of a dark pigment in hepatocytes, so that the liver of an affected individual appears dark blue or black [31, 145, 158]. The incidence of Dubin–Johnson syndrome ranges from 1:1,300 among Iranian Jews [152] to 1:300,000 in a Japanese population [70].
Sequence variants of the human ABCC2 gene identified in patients with Dubin–Johnson syndrome include splice-site mutations resulting in exon loss and subsequent premature stop codons [70, 118, 168, 180], missense mutations [47, 111, 112, 117, 166, 168, 180, 181], a deletion mutation leading to the loss of two amino acids from the second nucleotide-binding domain [170], a deletion/in-frame insertion mutation [16], and nonsense mutations leading to premature stop codons [134, 154, 166] (Table 3, Fig. 2). Although all sequence variants associated with Dubin–Johnson syndrome result in the absence of a functionally active ABCC2 protein from the canalicular membrane, their effects on the synthesis and function of the ABCC2 protein differ. Premature stop codons may cause rapid degradation of the mutated ABCC2 mRNA by nonsense-mediated decay, a mechanism which cotranslationally recognizes when a stop codon precedes the last splice-site [167], thus leading to the absence of the ABCC2 protein in some cases of Dubin–Johnson syndrome. In fact, a truncated ABCC2 protein has not been detected so far [134, 170]. Other Dubin–Johnson syndrome-associated sequence variants lead to deficient maturation and impaired sorting of the ABCC2 protein or to an apically localized, but functionally inactive, ABCC2 protein [47, 79, 80, 117] (Table 3).
Many naturally occurring sequence variants have been identified whose consequences on ABCC2 function are not yet known (Table 3). The potential effects of these single amino acid substitutions can be tentatively assessed by the online tool Polymorphism Phenotyping (PolyPhen, http://genetics.bwh.harvard.edu/pph [138]). Interestingly, all amino acid substitutions causing Dubin–Johnson syndrome are classified in this database as “probably damaging” (Table 3). However, the PolyPhen tool cannot replace the experimental analysis of each individual amino acid substitution to proof changes of ABCC2 function, as exemplified by some published transfection studies [79, 80, 117].
Two different mutant hyperbilirubinemic rat strains, the GY/TR− rats [64] and the Eisai hyperbilirubinemic rats (EHBR) [54, 163], have a hereditary defect in the secretion of anionic conjugates into bile. In consequence of the molecular identification and cloning of rat Abcc2 as the apical conjugate efflux pump [13, 60, 133], which is defective in these rat strains [13, 60, 133], both can now be considered as animal models of human Dubin–Johnson syndrome. Two distinct naturally occurring sequence variants in the rat Abcc2 gene, either at codon 401 (GY/TR−) [133] or at codon 855 (EHBR) [60], lead to premature stop codons and to a lack of the Abcc2 protein from the hepatocyte canalicular membrane [13, 114, 133]. Recently, Abcc2-knock-out mice have been generated and characterized by several groups [18, 121, 177]. These Abcc2-deficient mice are apparently healthy and fertile, as are the Abcc2-deficient rats. It will be of interest to crossbreed these mutants with mice lacking other Abc transporters, such as knock-out mice lacking Abcc3, Abcc4, Abcb1a and Abcb1b (Mdr1a and Mdr1b P-glycoprotein), and Abcg2 (Bcrp1).
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Acknowledgements
The work in the authors’ laboratory was supported by the German Cancer Research Center, by the Deutsche Forschungsgemeinschaft, Bonn, Germany, and the Wilhelm Sander–Stiftung, München, Germany. We acknowledge the contributions to this work by past and present members of the Division of Tumor Biochemistry, particularly from Jörg König, Markus Büchler, Yunhai Cui, Verena Keitel, Rosemarie Mayer, Wolfgang Hagmann, Manuela Brom, Hiroyuki Tsujii, Daniel Rost, Thomas Schaub, Kathrin Kopplow-de Jong, Inka Leier, and Gabriele Jedlitschky, as well as the collaboration with Jürgen Kartenbeck and Herbert Spring from the Cell Biology Division of the German Cancer Research Center.
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Nies, A.T., Keppler, D. The apical conjugate efflux pump ABCC2 (MRP2). Pflugers Arch - Eur J Physiol 453, 643–659 (2007). https://doi.org/10.1007/s00424-006-0109-y
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DOI: https://doi.org/10.1007/s00424-006-0109-y