Skip to main content

Advertisement

SpringerLink
Log in

Feasibility study of novel endoscopic Cerenkov luminescence imaging system in detecting and quantifying gastrointestinal disease: first human results

  • Molecular Imaging
  • Published:
European Radiology Aims and scope Submit manuscript

Abstract

Objectives

Cerenkov luminescence imaging (CLI) provides potential to use clinical radiotracers for optical imaging. The goal of this study was to present a newly developed endoscopic CLI (ECLI) system and illustrate its feasibility and potential in distinguishing and quantifying cancerous lesions of the GI tract.

Methods

The ECLI system was established by integrating an electron-multiplying charge-coupled device camera with a flexible fibre endoscope. Phantom experiments and animal studies were conducted to test and illustrate the system in detecting and quantifying the presence of radionuclide in vitro and in vivo. A pilot clinical study was performed to evaluate our system in clinical settings.

Results

Phantom and mice experiments demonstrated its ability to acquire both the luminescent and photographic images with high accuracy. Linear quantitative relationships were also obtained when comparing the ECLI radiance with the radiotracer activity (r 2 = 0.9779) and traditional CLI values (r 2 = 0.9025). Imaging of patients revealed the potential of ECLI in the identification and quantification of cancerous tissue from normal, which showed good consistence with the clinical PET examination.

Conclusions

The new ECLI system shows good consistence with the clinical PET examination and has great potential for clinical translation and in aiding detection of the GI tract disease.

Key Points

• CLI preserves the characteristics of both optical and radionuclide imaging.

• CLI provides great potential for clinical translation of optical imaging.

• The newly developed endoscopic CLI (ECLI) has quantification and imaging capacities.

• GI tract has accessible open surfaces, making ECLI a potentially suitable technique.

• Cerenkov endoscopy has great clinical potential in detecting GI disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

CLI:

Cerenkov luminescence imaging

CLT:

Cerenkov luminescence tomography

ECLI:

Endoscopic Cerenkov luminescence imaging

EMCCD:

Electron-multiplying charge-coupled device

GI:

Gastrointestinal

References

  1. Ruggiero A, Holland JP, Lewis JS, Grimm J (2010) Cerenkov luminescence imaging of medical isotopes. J Nucl Med 51:1123–1130

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Spinelli AE, D’Ambrosio D, Calderan L, Marengo M, Sbarbati A, Boschi F (2010) Cerenkov radiation allows in vivo optical imaging of positron emitting radiotracers. Phys Med Biol 55:483

    Article  PubMed  Google Scholar 

  3. Xu Y, Liu H, Cheng Z (2011) Harnessing the power of radionuclides for optical imaging: Cerenkov luminescence imaging. J Nucl Med 52:2009–2018

    Article  PubMed  Google Scholar 

  4. Jelley J (1955) Cerenkov radiation and its applications. Br J Appl Phys 6:227

    Article  Google Scholar 

  5. Robertson R, Germanos MS, Li C, Mitchell GS, Cherry SR, Silva MD (2009) Optical imaging of Cerenkov light generation from positron-emitting radiotracers. Phys Med Biol 54:N355–N365

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Spinelli AE, Ferdeghini M, Cavedon C et al (2013) First human Cerenkography. J Biomed Opt 18:020502

    Article  Google Scholar 

  7. Boschi F, Calderan L, D’Ambrosio D et al (2011) In vivo 18F-FDG tumour uptake measurements in small animals using Cerenkov radiation. Eur J Nucl Med Mol Imaging 38:120–127

    Article  PubMed  Google Scholar 

  8. Hu Z, Ma X, Qu X et al (2012) Three-dimensional noninvasive monitoring iodine-131 uptake in the thyroid using a modified Cerenkov luminescence tomography approach. PLoS ONE 7:e37623

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Jeong SY, Hwang M-H, Kim JE et al (2011) Combined Cerenkov luminescence and nuclear imaging of radioiodine in the thyroid gland and thyroid cancer cells expressing sodium iodide symporter: initial feasibility study. Endocr J 58:575

    Article  CAS  PubMed  Google Scholar 

  10. Robertson R, Germanos MS, Manfredi MG, Smith PG, Silva MD (2011) Multimodal imaging with 18F-FDG PET and Cerenkov luminescence imaging after MLN4924 treatment in a human lymphoma xenograft model. J Nucl Med 52:1764–1769

    Article  PubMed  Google Scholar 

  11. Li C, Mitchell GS, Cherry SR (2010) Cerenkov luminescence tomography for small animal imaging. Opt Lett 35:1109–1111

    Article  PubMed Central  PubMed  Google Scholar 

  12. Hu Z, Liang J, Yang W et al (2010) Experimental Cerenkov luminescence tomography of the mouse model with SPECT imaging validation. Opt Express 18:24441–24450

    Article  CAS  PubMed  Google Scholar 

  13. Liu H, Carpenter CM, Jiang H et al (2012) Intraoperative imaging of tumors using Cerenkov luminescence endoscopy: a feasibility experimental study. J Nucl Med 53:1579–1584

    Article  PubMed  Google Scholar 

  14. Kothapalli S-R, Liu H, Liao JC, Cheng Z, Gambhir SS (2012) Endoscopic imaging of Cerenkov luminescence. Biomed Opt Express 3:1215

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Ma X, Yang W, Zhou S et al (2012) Study of penetration depth and resolution of Cerenkov luminescence emitted from (18)F-FDG and (131)I. J Nucl Med 53:55

    Article  Google Scholar 

  16. China Food and Drug Administration, imported instrument database history (2006) http://app1.sfda.gov.cn/datasearch/face3/dir.html. Accessed 5 Apr 2014

  17. Thorek DL, Ogirala A, Beattie BJ, Grimm J (2013) Quantitative imaging of disease signatures through radioactive decay signal conversion. Nat Med 19:1345–1350

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Chen X, Gao X, Chen D et al (2010) 3D reconstruction of light flux distribution on arbitrary surfaces from 2D multi-photographic images. Opt Express 18:19876–19893

    Article  PubMed  Google Scholar 

  19. Dothager RS, Goiffon RJ, Jackson E, Harpstrite S, Piwnica-Worms D (2010) Cerenkov radiation energy transfer (CRET) imaging: a novel method for optical imaging of PET isotopes in biological systems. PLoS ONE 5:e13300

    Article  PubMed Central  PubMed  Google Scholar 

  20. Liu H, Zhang X, Xing B, Han P, Gambhir SS, Cheng Z (2010) Radiation‐luminescence‐excited quantum dots for in vivo multiplexed optical imaging. Small 6:1087–1091

    Article  CAS  PubMed  Google Scholar 

  21. Carpenter CM, Sun C, Pratx G, Liu H, Cheng Z, Xing L (2012) Radioluminescent nanophosphors enable multiplexed small-animal imaging. Opt Express 20:11598

    Article  PubMed Central  PubMed  Google Scholar 

  22. Luo H, Zhang L, Liu X et al (2013) Water exchange enhanced cecal intubation in potentially difficult colonoscopy. Unsedated patients with prior abdominal or pelvic surgery: a prospective, randomized, controlled trial. Gastrointest Endosc 77:767–773

    Article  PubMed  Google Scholar 

  23. Xu Y, Chang E, Liu H, Jiang H, Gambhir SS, Cheng Z (2012) Proof-of-concept study of monitoring cancer drug therapy with cerenkov luminescence imaging. J Nucl Med 53:312–317

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  24. Thorek DL, Riedl C, Grimm J (2014) Clinical Cerenkov luminescence imaging of 18F-FDG. J Nucl Med 55:95–98

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The scientific guarantor of this publication is Xueli Chen at Xidian University. The authors would like to thank Dr. Xiaowei Ma from Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University for the technical assistance. The authors of this manuscript declare no relationships with any companies whose products or services may be related to the subject matter of the article. This work was supported in part by the National Natural Science Foundation of China under Grant Nos. 81090270, 81090272, 81090273, 81101083, 81230033, 81371615 and 81370585; Program of the National Basic Research and Development Program of China (973) under Grant Nos. 2011CB707702, 2010CB529302, the National Municipal Science and Technology Project under Grant Nos. 2009ZX09103-667, 2009ZX09301-009-RC06, and the Open Research Project under Grant 20120101 from SKLMCCS. No complex statistical methods were necessary for this paper. Institutional review board approval was obtained. Written informed consent was obtained from all subjects (patients) in this study. Approval from the institutional animal care committee was obtained. Methodology: experimental, performed at one institution.

Author information

Author notes

    Authors and Affiliations

    1. State Key Laboratory of Cancer Biology, Department of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

      Hao Hu, Shujun Li, Liping Yao, Jie Liang, Yongzhan Nie & Kaichun Wu

    2. School of Life Science and Technology, Xidian University, Xi’an, 710071, China

      Xin Cao, Yenan Lin, Muhan Liu, Jimin Liang & Xueli Chen

    3. Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

      Fei Kang & Jing Wang

    4. Department of Gastroenterology, Xi’an Children’s Hospital, Xi’an, China

      Min Wang

    Authors
    1. Hao Hu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Xin Cao
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Fei Kang
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Min Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Yenan Lin
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Muhan Liu
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Shujun Li
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Liping Yao
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Jie Liang
      View author publications

      You can also search for this author in PubMed Google Scholar

    10. Jimin Liang
      View author publications

      You can also search for this author in PubMed Google Scholar

    11. Yongzhan Nie
      View author publications

      You can also search for this author in PubMed Google Scholar

    12. Xueli Chen
      View author publications

      You can also search for this author in PubMed Google Scholar

    13. Jing Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    14. Kaichun Wu
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding authors

    Correspondence to Xueli Chen, Jing Wang or Kaichun Wu.

    Additional information

    Hao Hu, Xin Cao and Fei Kang contributed equally to this work.

    Electronic supplementary material

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Hu, H., Cao, X., Kang, F. et al. Feasibility study of novel endoscopic Cerenkov luminescence imaging system in detecting and quantifying gastrointestinal disease: first human results. Eur Radiol 25, 1814–1822 (2015). https://doi.org/10.1007/s00330-014-3574-2

    Download citation

    • Received:

    • Revised:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00330-014-3574-2

    Keywords

    Search

    Navigation