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Comparing amyloid-β deposition, neuroinflammation, glucose metabolism, and mitochondrial complex I activity in brain: a PET study in aged monkeys

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Abstract

Purpose

The aim of the present study was to compare amyloid-β (Aβ) deposition, translocator protein (TSPO) activity, regional cerebral metabolic rate of glucose (rCMRglc), and mitochondrial complex I (MC-I) activity in the brain of aged monkeys.

Methods

PET scans with 11C-PIB (Aβ), 18F-BCPP-EF (MC-I), 11C-DPA-713 (TSPO), and 18F-FDG (rCMRglc) were performed in aged monkeys (Macaca mulatta) in the conscious state and under isoflurane anaesthesia. 11C-PIB binding to Aβ and 11C-DPA-713 binding to TSPO were evaluated in terms of standard uptake values (SUV). The total volume of distribution (V T) of 18F-BCPP-EF and rCMRglc with 18F-FDG were calculated using arterial blood sampling.

Results

Isoflurane did not affect MC-I activity measured in terms of 18F-BCPP-EF uptake in living brain. There was a significant negative correlation between 18F-BCPP-EF binding (V T) and 11C-PIB uptake (SUVR), and there was a significant positive correlation between 11C-DPA-713 uptake (SUV) and 11C-PIB uptake. In contrast, there was no significant correlation between rCMRglc ratio and 11C-PIB uptake.

Conclusion

18F-BCPP-EF could be a potential PET probe for quantitative imaging of impaired MC-I activity that is correlated with Aβ deposition in the living brain.

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Acknowledgements

We gratefully acknowledge the technical assistance provided by Kaori Suzuki and Aiko Iwazaki.

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Authors and Affiliations

  1. Central Research Laboratory, Hamamatsu Photonics K.K., 5000 Hirakuchi, Hamakita, Shizuoka, 434-8601, Japan

    Hideo Tsukada, Shingo Nishiyama, Hiroyuki Ohba, Masakatsu Kanazawa, Takeharu Kakiuchi & Norihiro Harada

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  1. Hideo Tsukada
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  2. Shingo Nishiyama
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  3. Hiroyuki Ohba
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  4. Masakatsu Kanazawa
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  5. Takeharu Kakiuchi
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Correspondence to Hideo Tsukada.

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Tsukada, H., Nishiyama, S., Ohba, H. et al. Comparing amyloid-β deposition, neuroinflammation, glucose metabolism, and mitochondrial complex I activity in brain: a PET study in aged monkeys. Eur J Nucl Med Mol Imaging 41, 2127–2136 (2014). https://doi.org/10.1007/s00259-014-2821-8

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  • DOI: https://doi.org/10.1007/s00259-014-2821-8

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