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Simultaneous in vivo magnetic resonance imaging and radioactive measurements with the β-MicroProbe

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European Journal of Nuclear Medicine and Molecular Imaging Aims and scope Submit manuscript

Abstract

Purpose

Multimodal instrumentation is a new technical approach allowing simultaneous and complementary in vivo recordings of complementary biological parameters. To elucidate further the physiopathological mechanisms in intact small animal models, especially for brain studies, a challenging issue is the actual coupling of magnetic resonance imaging (MRI) techniques with positron emission tomography (PET): it has been shown that running the technology for radioactive imaging in a magnet alters the spatiotemporal performance of both modalities. Thus, we propose an alternative coupling of techniques that uses the β-MicroProbe instead of PET for local measurements of radioactivity coupled with MRI.

Methods

We simultaneously recorded local radioactivity due to [18F]MPPF (a 5-HT1A receptor PET radiotracer) binding in the hippocampus with the β-MicroProbe and carried out anatomical MRI in the same anaesthetised rat.

Results

The comparison of [18F]MPPF kinetics obtained from animals in a magnet with kinetics from a control group outside the magnet allowed us to determine the stability of tracer biokinetic measurements over time in the magnet. We were thus able to show that the β-MicroProbe reliably measures radioactivity in rat brains under an intense magnetic field of 7 Tesla.

Conclusion

The biological validation of a β-MicroProbe/MRI dual system reported here opens up a wide range of future multimodal approaches for functional and pharmacological measurements by the probe combined with various magnetic resonance technologies, including anatomical MRI, functional MRI and MR spectroscopy.

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References

  1. Goertzen A, Meadors A, Silverman R, Cherry SR. Simultaneous molecular and anatomical imaging of the mouse in vivo. Phys Med Biol 2002;47:4315–28.

    Article  PubMed  Google Scholar 

  2. Marsden PK, Strul D, Keevil SF, William SC, Cash D. Simultaneous PET and NMR. Br J Radiol 2002;75:S53–9.

    PubMed  Google Scholar 

  3. Cherry SR. Mulitmodality in vivo imaging systems: twice the power or double the trouble? Annu Rev Biomed Eng 2006;8:35–62.

    Article  PubMed  CAS  Google Scholar 

  4. Pichler B, Judenhofer M, Catana C, Walton J, Kneilling M, Nutt R, et al. Performance test of an LSO-APD detector in a 7-T MRI scanner for simultaneous PET/MRI. J Nucl Med 2006;47:639–47.

    PubMed  Google Scholar 

  5. Pain F, Laniece P, Mastrippolito R, Charon Y, Comar D, Leviel V, et al. SIC, an intracerebral radiosensitive probe for in vivo neuropharmacology investigations in small laboratory animals : first prototype design characterisation and in vivo evaluation. IEEE TNS 2002;49:822–6.

    CAS  Google Scholar 

  6. Desbree A, Pain FP, Gurden H, Pinot L, Grenier D, Zimmer L, et al. A new multimodality system for quantitative in vivo studies in small animals: combination of nuclear magnetic resonance and the radiosensitive beta-MicroProbe. IEEE TNS 2005;52:1281–7.

    Google Scholar 

  7. Plenevaux A, Weissmann D, Aerts J, Lemaire C, Brihaye C, Degueldre C, et al. Tissue distribution, autoradiography, and metabolism of 4-(2′-methoxyphenyl)-1-[2′-[N-2″-pyridinyl)-p-[18F]fluorobenzamido]ethyl]piperazine (p-[18F]MPPF), a new serotonin 5-HT1A antagonist for positron emission tomography: an in vivo study in rats. J Neurochem 2000;75:803–11.

    Article  PubMed  CAS  Google Scholar 

  8. Paxinos G, Watson C. The rat stereotaxic coordinates. 4th ed. San Diego: Academic Press; 1998.

    Google Scholar 

  9. Rbah L, Leviel V, Zimmer L. Displacement of the PET ligand [18F]MPPF by the electrically-evoked serotonin release in the rat hippocampus. Synapse 2003;49:239–45.

    Article  PubMed  CAS  Google Scholar 

  10. Catana C, Wu Y, Judenhofer MS, Qi J, Pichler BJ, Cherry S. Simultaneous acquisition of multislice PET and MR images: initial results with a MR-compatible PET scanner. J Nucl Med 2006;47:1968–76.

    PubMed  Google Scholar 

  11. Desbrée A, Pain F, Gurden H, Zimmer L, Pinot L, Lanièce P, et al. Combining the radiosensitive Beta MicroProbe to nuclear magnetic resonance: theoretical approach for in vivo studies in small animals. J Neurosci Methods 2004;140:47–52.

    Article  PubMed  Google Scholar 

  12. Zimmer L, Mauger G, Le Bars D, Bonmarchand G, Luxen A, Pujol J. Effect of endogeneous serotonin on the binding of the 5-HT1A PET ligand 18F-MPPF in the rat hippocampus: kinetic Beta measurements combined with microdialysis. J Neurochem 2002;80:278–86.

    Article  PubMed  CAS  Google Scholar 

  13. Attwell D, Iadecola C. The neural basis of functional brain imaging signals. Trends Neurosci 2002;25:621–5.

    Article  PubMed  CAS  Google Scholar 

  14. Boumezbeur F, Besret L, Valette J, Gregoire M, Delzescaux T, Maroy R, et al. Glycolysis versus TCA cycle in the primate brain as measured by combining 18F-FDG PET and 13C-NMR. J Cereb Blood Flow Metab 2005;25:1418–23.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank Rhône-Alpes Genopole and Fondation Rhône-Alpes Futur funded by the Réseau National des Genopoles.

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Correspondence to A. Desbrée.

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Desbrée, A., Rbah, L., Langlois, JB. et al. Simultaneous in vivo magnetic resonance imaging and radioactive measurements with the β-MicroProbe. Eur J Nucl Med Mol Imaging 34, 1868–1872 (2007). https://doi.org/10.1007/s00259-007-0475-5

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  • DOI: https://doi.org/10.1007/s00259-007-0475-5

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