Abstract
Purpose
The purpose of this study was to visualise experimental atherosclerotic lesions using radiolabelled antisense oligonucleotides (ASONs).
Methods
Atherosclerosis was induced in New Zealand White rabbits fed 1% cholesterol for approximately 60 days. In vivo and ex vivo imaging was performed in atherosclerotic rabbits and normal control rabbits after i.v. injection of 92.5±18.5 MBq 99mTc-labelled ASON or 99mTc-labelled sense oligonucleotides. Immediately after the in vivo imaging, the animals were sacrificed and ex vivo imaging of the aortic specimens was performed. Biodistribution of radiolabelled c-myc ASON was evaluated in vivo in atherosclerotic rabbits.
Results
Planar imaging revealed accumulation of 99mTc-labelled c-myc ASON in atherosclerotic lesions along the artery wall. Ex vivo imaging further demonstrated that the area of activity accumulation matched the area of atherosclerotic lesions. In contrast, no atherosclerotic lesions were found in the vessel wall and no positive imaging results were obtained in animals of the control group.
Conclusion
This molecular imaging approach has potential for non-invasive imaging of atherosclerotic plaques at an early stage.
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Acknowledgements
The authors are grateful to Dr. Donald J. Hnatowich from the University of Massachusetts Medical Center, USA, for providing the MAG3 chelator. This work was supported by grant No. 30070310 from the National Natural Science Foundation of China.
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Qin, G., Zhang, Y., Cao, W. et al. Molecular imaging of atherosclerotic plaques with technetium-99m-labelled antisense oligonucleotides. Eur J Nucl Med Mol Imaging 32, 6–14 (2005). https://doi.org/10.1007/s00259-004-1700-0
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DOI: https://doi.org/10.1007/s00259-004-1700-0